益髓颗粒对免疫性血小板减少性紫癜模型小鼠细胞相关因子表达影响  

Effects of Yisui Granule on cytokines expression immune thrombocytopenic purpura mouse model

作　　者：吴晓勇[1] 李冬云[1] 陈信义[1] 王瑞昙[2] 岳明[2] 张恩户[2] 

机构地区：[1]北京中医药大学东直门医院肿瘤血液科,北京100700 [2]陕西中医学院药理教研室,咸阳712046

出　　处：《中华中医药杂志》2010年第6期849-852,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药管理局课题(No.06-07JP01)~~

摘　　要：目的:建立免疫性血小板减少性紫癜(ITP)小鼠动物模型,观察益髓颗粒对模型小鼠外周血细胞因子影响,探讨益髓颗粒的免疫调节机制。方法:40只Balb/c小鼠随机分为正常组、模型组、泼尼松治疗组和益髓颗粒治疗组,除正常组外,其余各组隔日1次腹腔注射豚鼠抗小鼠血小板血清(GP-APS)建立ITP小鼠模型,于造模第8d开始,各组均按0.2mL/(10g·d)体积灌胃。其中正常组、模型组灌服生理盐水;泼尼松按0.0113mg/(10g·d)灌胃;益髓颗粒按0.3g/(10g·d)灌胃。连续7d后眼球取血分离血清,酶联免疫吸附法(ELISA)检测干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)。结果:与正常组相比,模型组小鼠IFN-γ水平明显升高,IL-4水平明显降低,IFN-γ/IL-4比值明显异常;与模型组相比,泼尼松治疗、益髓颗粒治疗组的IFN-γ、IL-4均有所恢复,IFN-γ/IL-4比值接近正常。结论:益髓颗粒可通过影响Th1/Th2细胞及其因子水平而对ITP免疫功能起调节作用。Objective：To observe the effects of Yisui Granule on peripheral blood cytokines in immune thrombocytopenic purpura （ITP） mouse model and discuss it＇s immune regulatory mechanism. Methods：40 Balb/c mice were randomly divided into normal group,model group,prednisone acetate group and Yisui Granule group. ITP mouse model were made by injected intraperitoneally guinea pig-antimouse platelet serum（GP-APS） every other day. On the 8th day establishing the model,each group were inrtagastrically at a dose of 0.2mL/（10g·d） volume. Normal group and model group were fed with normal saline,prednisone acetate and Yisui Granule were inrtagastrically administered for seven days at a dose of 0.0113mg/（10g·d）,0.3g/（10g·d） respectively. The levels of IFN-γ and IL-4 in peripheral blood serum were detected by ELISA. Results：The level of IFN-γ was significantly higher in model group than that in normal group,and the levels IL-4 decreased in model group,the ratio of IFN-γ/IL-4 increased. Compared with model group,the levels of IFN-γ and the ratio of IFN-γ/IL-4 decreased,the levels of IL-4 increased in prednisone acetate group and Yisui Granule group. Conclusion：Yisui Granule can regulate the immune function of ITP by influencing the level of Th1/Th2 cells and their cytokines.

关 键 词：益髓颗粒 免疫性血小板减少性紫癜 干扰素-Γ 白细胞介素-4 

分 类 号：R285.5[医药卫生—中药学]