化学发光法检测梅毒螺旋体特异性抗体的实验评价  

Experimental evaluation of the determination of specific antibodies to Treponema pallidum by chemiluminescent immunoassay

作　　者：侯晓菁[1] 梁艳[1] 陈洁[1] 孙贞[1] 崔艳芳[1] 仲人前[1] 王皓[1] 

机构地区：[1]第二军医大学附属长征医院实验诊断科,上海200003

出　　处：《检验医学》2010年第5期365-367,共3页Laboratory Medicine

摘　　要：目的应用免疫印迹法(WB)比较临床常用的梅毒螺旋体明胶凝集试验(TPPA)与化学发光法(CLIA)检测血清梅毒螺旋体特异性抗体的符合率。方法收集7 805份临床检测的血清标本,分别用TPPA和CLIA进行梅毒螺旋体特异性抗体检测,对结果不一致的标本应用WB检测确证。结果 7 805份血清标本中,CLIA检测阳性310例,TPPA检测阳性262例。对结果不一致的48例标本应用WB检测验证,结果证实阳性36例,临界阳性8例,阴性4例,而TPPA的结果均为阴性。TPPA检测总符合率为99.44%,CLIA检测总符合率为99.95%。结论 CLIA敏感性优于临床常用的TPPA,且具有结果客观、易分析、重复性好等优点,但也存在个别假阳性和钩状效应,因此应结合TPPA及临床资料确诊。Objective To compare the consistency of Treponema pallidum particle agglutination（TPPA） and chemiluminescent immunoassay（CLIA） in detecting the specific antibody against Treponema pallidum in serum by western blotting（WB）.Methods 7 805 serum samples were collected and detected by TPPA and CLIA.The samples with different results were determined by WB.Results In 7 805 serum samples,310 were positive for CLIA,while 262 were positive for TPPA.48 serum samples with contradictory results were determined by WB,and the results showed that 36 were positive,8 were borderline-positive and 4 were negative.However,the results of TPPA were all negative.The consistency of TPPA was 99.44%,while the consistency of CLIA was 99.95%.Conclusions The sensitivity of CLIA is better than that of TPPA and it has the advantages of objectivity,reproducibility and easy-to-analysis,but the false positive rate and hook effect should be paid attention to.As a result,CLIA should be combined with TPPA and confirmed by clinical information.

关 键 词：化学发光法 梅毒螺旋体明胶凝集试验 免疫印迹法 梅毒螺旋体特异性抗体 

分 类 号：R377[医药卫生—病原生物学]