薄壳型手性配体交换HPLC填料的合成及用于DL-氨基酸拆分  

SYNTHESIS OF SURFACE-COATED CHIRAL LIGAND EXCHANGE HPLC PACKING AND ITS APPLICATION IN RESOLUTION OF DLAMINO ACIDS

作　　者：阎虎生[1] 王东[1] 李文兰[1] 程晓辉[1] 何炳林[1] 

机构地区：[1]南开大学高分子化学研究所,吸附与分离功能高分子材料国家重点实验室,天津300071

出　　处：《离子交换与吸附》1999年第1期86-89,共4页Ion Exchange and Adsorption

摘　　要：将含有少量二乙烯苯的丙烯酸甲酯涂敷于聚二乙烯苯珠体表面上进行聚合，和到了表面涂层树脂，通过红外光谱估算出涂层厚度为－２μｍ。涂层树脂依次与乙醇胺，环氧氯丙烷一Ｌ－脯氨酸反应，得到薄壳型手性配体交换树脂，此树脂的Ｃｕ络合物作为高效液相色谱的填料对ＤＬ－氨基酸进行了拆分。Methyl acrylate with a small amount of divinylbenzene was coated and polymerized on the external surface of polydivinylbenzene beads, obtaining surface-coated resin. The thickness of the coating layer was estimated to be ~2μ m by infrared spectra. The coated resin was reacted, respectively, with ethanolamine, epichlorohydrin and L-proline to form chiral ligand exchange coating resin. The resin, coordinated with Cu(Ⅱ), was used as HPLC packing to resolve DL-amino acids.

关 键 词：配位交换色谱 氨基酸 填料 HPLC 拆分 涂层 

分 类 号：Q517.03[生物学—生物化学] O657.720.3[理学—分析化学]