人ⅡA型磷脂酶A_2的cDNA顺序与婴儿利什曼原虫DNA相似性分析  

ANALYSIS OF CONSENSUS SEQUENCES BETWEEN HOMO SAPIENS PHOSPHOILIPASE A_2 cDNA AND LEISHMANIA INFANTUM DNA

作　　者：何睿林[1] 梁宁生[2] 

机构地区：[1]广西医科大学第一附属医院 [2]广西医科大学附属肿瘤医院,南宁530021

出　　处：《广西医科大学学报》2010年第2期173-176,共4页Journal of Guangxi Medical University

基　　金：广西科学基金资助项目(No.桂科回0639026);广西卫生厅重点资助项目(No.重200707)

摘　　要：目的:了解人ⅡA型磷脂酶A2cDNA顺序和婴儿利什曼原虫DNA相似性的情况和原因。方法:利用BLAST工具,以人ⅡA型磷脂酶cDNA顺序中的片段对核酸数据库进行搜寻和比对,将获得的一致性片段进行收集、整理并分析、汇总利什曼原虫DNA的相关信息。结果:发现人ⅡA型磷脂酶A2cDNA与婴儿利什曼原虫DNA之间具有9个完全一致序列(≥16bp),这些序列集中分布于利什曼原虫36条染色体的29、30号染色体上。结论:人ⅡA型磷脂酶cDNA顺序与婴儿利什曼原虫DNA片段之间具有一致性,可能与基因由原虫向人的横向传递有关。Objective：To analyse the similarity between Homo sapiens phosphoilipase A2 cDNA and Leishmania infantum DNA and to understand the cause. Methods：By the tools of BLAST,aligned the gene fragments from Homo sapiens phosphoilipase A2 cDNA to nucleartide database step by step, collecting and sorting all of the consensus sequences provide to analysis. Results：There were 9 consensus sequences between Homo sapiens phosphoilipase A2 eDNA and Leishmania infantum DNA（〉16 bp）, and the sequences distribut mainly in the chromesome 29 and ehromesome 30. Conclusion： There are some indenty DNA fragments between Homo sapiens phosphoilipase A2 and Leishmania infantum DNA, it may due to horizontal transfer from Leishmania to Homo sapiens.

关 键 词：磷脂酶A2 人 利什曼原虫 分子进化 横向传递 

分 类 号：Q342.3[生物学—遗传学]