乙肝病毒携带者PBMC细胞中Toll样受体2表达水平与乙肝病毒增殖相关性研究  

THE STUDY ON RELATIONSHIP BETWEEN TLR2 IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF ASC AND PROLIFERATION OF HBV

作　　者：冷静[1] 吴亚滨[1] 郑禹[1] 王启辉[1] 李佳荃[1] 白玉[1] 

机构地区：[1]广西医科大学免疫学教研室,南宁530021

出　　处：《广西医科大学学报》2010年第2期224-227,共4页Journal of Guangxi Medical University

基　　金：广西青年自然科学基金(桂科青0135012);广西医科大学博士启动基金资助项目

摘　　要：目的:探讨乙肝病毒(HBV)增殖与无症状乙肝病毒携带者(ASC)外周血中Toll样受体2(TLR2)表达水平的相关性。方法:分离乙肝病毒携带者和健康志愿者外周血单个核细胞,分别用免疫细胞化学染色方法检测TLR2在细胞表面的表达情况;用RT-PCR方法检测TLR2的mRNA水平;用ELISA法检测乙肝病毒携带者和健康志愿者血清中TLR2活化后产物TNF-α的表达水平。结果:HBV复制活跃组的外周血单个核细胞中TLR2在蛋白水平和mRNA水平表达明显低于HBV复制不活跃组和健康对照组;HBV复制活跃组血清中TNF-α水平显著低于HBV复制不活跃组。结论:乙肝病毒携带者外周血单个核细胞TLR2的表达及活化与乙肝病毒的增殖有相关性。Objective：To study the relationship between the expression level of Toll-like receptor on surface of peripheral blood mononuclear cells（PMBCs）of asymptomatic HBV carrier（ASC）and the proliferation of HBV.Methods：PMBCs and sera from ASC and health people were isolated and collected.The expression of TLR2 in the surface of PMBCs and the level of TLR2 mRNA transcription in PMBCs were detected by immunocytochemistry and RT-PCR,respectively.An ELISA Kit was used to detect serum TNF-α level.Results：The intensity of TLR2 expression on surface of PMBCs and the level of TLR2 mRNA transcription in PMBCs in high level of HBV copy group were significantly lower than those in health control group and low level of HBV copy group.The intensity of serum TNF-α in low level of HBV copy group is significantly higher than that in high level of HBV copy group.Conclusion：The expression and activation of TLR2 on PBMCs of ASC correlate significantly with the HBV proliferation.

关 键 词：Toll样受体 乙肝病毒 外周血单个核细胞 无症状乙肝病毒携带者 

分 类 号：R512.62[医药卫生—内科学]