‘甜查理’草莓花药培养脱毒技术  

Studies on Anther Culture of 'Sweet Charlie' Strawberry

作　　者：晁慧娟[1] 刘敏[1] 姬谦龙[2] 高遐虹[1] 董清华[1] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京农学院组培实验中心,北京102206

出　　处：《北京农学院学报》2010年第2期18-21,共4页Journal of Beijing University of Agriculture

基　　金：北京市教委科技发展计划项目(KM200710020012);昌平区科委科技发展计划项目

摘　　要：本试验以花粉发育处于单核靠边期的‘甜查理’草莓花蕾为外植体进行花药脱毒培养。结果表明:预冷处理72 h是最适合的花药低温处理时间。最适合花药愈伤组织的诱导培养基是MS+6-BA1.0 mg/L+2,4-D2.0 mg/L,出愈率为70.9%;最适合的不定芽分化培养基是MS+6-BA1.0 mg/L+NAA0.1 mg/L,分化率为38.0%。The callus inducing rate was increased significantly and the quality of the callus was the best when the anthers at the late uninucleate stage were pretreated in 4 ℃ for 72 hours. The optimal callus inducing medium for Sweet Charlie＇ strawberry anthers was MS＋6-BA 1.0 rag/L＋2,4-D 2. 0 mg/L, the callus induction rate was 70. 9 %; 0. 1 mg/L, the differentiation rate The optimal differentiation medium was MS＋6-BA 1.0 mg/L＋ NAA was 38.0%.

关 键 词：草莓 甜查理 花药培养 脱毒 

分 类 号：S668.4[农业科学—果树学]