黄芪总黄酮对辐射损伤小鼠的防护作用研究  

Research on the radioprotective effect of total astragalus flavonoids on the irradiated mice

作　　者：杨映雪[1] 陈建业[2] 费中海[3] 谭榜宪[4] 唐恩洁[5] 唐华英[2] 王亚平[1] 

机构地区：[1]重庆医科大学基础医学院组胚学教研室,重庆400016 [2]川北医学院应用技术研究所,南充637000 [3]川北医学院附属医院检验科,南充637000 [4]川北医学院附属医院放疗科,南充637000 [5]川北医学院免疫学与微生物学教研室,南充637000

出　　处：《重庆医科大学学报》2010年第4期504-507,共4页Journal of Chongqing Medical University

基　　金：四川省科技厅重点项目(编号:05SG1859);四川省科技厅应用基础项目(编号:2006J13-031)

摘　　要：目的:研究黄芪总黄酮(Total flavonoids of astragalus,TFA)对受60Coγ射线损伤小鼠的辐射防护作用,为TFA的临床应用提供实验依据。方法:采用大剂量60Coγ射线一次性全身照射雌性昆明小鼠,制成放射损伤动物模型。小鼠在照射前3d及照射后,以不同剂量的TFA连续灌胃14d,观察受致死剂量60Coγ射线照射小鼠的30d存活率及生存时间长短。同时,在低剂量照射组检测用药后小鼠体重、外周血指标、脾脏指数的变化。用HE染色观察脾脏的病理学改变。结果:TFA不仅能明显延长辐射损伤小鼠的平均存活天数,提高小鼠30d存活率(P<0.05);而且能显著降低辐射损伤小鼠外周血白细胞的下降幅度、缩短其恢复所需时间(P<0.05);同时,TFA还能促进小鼠脾重指数的恢复,减轻辐射对小鼠脾脏的损伤程度,促进造血灶增生,加快造血功能恢复。结论:TFA具有较强的抗辐射作用,能显著提高受致死剂量60Coγ射线照射小鼠的存活率,降低辐射对小鼠外周血WBC和PLT及脾脏的损伤作用,其具体作用机制有待进一步深入研究。Objective：To explore the radioprotective effect of TFA on the ^60Coγ-rayirradiated mice and provide an experimental basis for the clinical use of TFA.Methods：Normal female Kunming mice were intragastrically administered with TFA for 3days before exposure to lethal dose of ^60Coγ-ray radiation and then continuously administered with TFA for 11days.The 30-days survival rate and average life span of irradiated mice were estimated.Meanwhile the body weight,peripheral blood parameters,spleen indexes and the pathological changes of spleen were also observed in the sublethal dose irradiated mice.Results：TFA could not only increase the 30-days survival rate and the average survival days of dead mice（P〈0.05）,but also could cut down significantly the descent extent of the peripheral blood WBC numbers and shorten the time of WBC restoration rapidly inγ-ray irradiated mice（P〈0.05）.Furthermore TFA could also promote the recovery of spleen index,decrease the damage ofγ-ray on the spleen and promote the growth of hematopoietic cells and blood-producing function.Conclusion：TFA has significant anti-radiation effect and can effectively raise the survival rate of mice irradiated with a lethal dose of ^60Coγ-rays.It can also reduce the radiation injury of WBC and PLT as well as spleen in irradiated mice.

关 键 词：黄芪总黄酮 辐射损伤 辐射防护 

分 类 号：R285.5[医药卫生—中药学]