AQP8在正常人绒毛滋养层细胞与绒癌JAR细胞株中的表达  

Expression of AQP8 in cultured normal humantrophocyte and JAR cell line

作　　者：王莉[1] 张建华[1] 张华[1] 王思义[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《重庆医科大学学报》2010年第4期512-515,共4页Journal of Chongqing Medical University

基　　金：重庆市自然科学基金(编号:2004-5480)

摘　　要：目的:探讨AQP8在正常人绒毛滋养细胞和绒癌JAR细胞株中的表达差异,及其表达改变与绒癌发生的关系。方法:正常人绒毛滋养细胞(正常组)的原代培养、纯化及鉴定;人绒癌JAR细胞株(绒癌组)的培养。以免疫组织化学法、免疫荧光激光共聚焦和RT-PCR技术检测AQP8蛋白和AQP8 mRNA在正常人绒毛滋养细胞和绒癌JAR细胞株细胞膜上的表达。结果:(1)应用免疫组化方法,在正常组中,仅能从显微镜中肉眼看到AQP8的极低表达,摄影效果不佳,而在绒癌组中AQP8的阳性细胞率为93.27%±14.45%,远高于正常组。(2)应用免疫荧光染色后行激光共聚焦成像技术发现,在相同的条件及激发强度下,可见AQP8在绒癌组呈绿色阳性表达,而在正常组中未见绿光,但当加强激发强度后,仍可探及AQP8在正常组的表达。(3)应用RT-PCR技术,在正常组与绒癌组中,均检测到AQP8 mRNA的表达。绒癌组AQP8的OD值(0.98±0.35)明显高于正常组AQP8的OD值(0.32±0.04)(P<0.05)。结论:AQP8表达异常上调,滋养细胞内、外水、电解质平衡将被破坏,可能是导致绒癌发生的原因之一。Objective：To discuss the different expression of AQP8 in cultured normal human trophocyte and JAR cell line,and the relationship between the changed expression of the channel protein and nosogenesis of chorionic carcinoma.Methods：The human cultured normal trophocyte was primarily cultured,purified and identified.JAR cell line was cultured.The expression of AQP8 was detected in cultured normal human trophocyte and JAR cell line by immunohistochemistry immunofluorescence confocal laser and RT-PCR.Results：（1）In the control group,the limitied expression of AQP8 was observed in cultured normal human trophocyte and JAR cell line,but in the exprimental group,the rate of positive cell of AQP8 in JAR cell line were 93.27%±14.45%,much higher than that in the control group.（2）By immunofluorescence staining confocal laser imaging,it was revealed that,in the same condition and excitation intensity,AQP8 in the JAR choriocarcinoma cell lines showed positive expression of the green,while in normal trophoblast cells,no green light,but when the excitation intensity was strengthened,it may still explore AQP8 in normal human villous trophoblast cell expression（.3）The expression of AQP8mRNA was detected in both cultured normal human trophocyte and JAR cell line.The OD value（0.98±0.35）of AQP8 in the experimental group was significantly higher than that（0.32±0.04）in the control group.Conclusion：Abnormal overexpression of AQP8 will destroy the external water and electrolyte balance of trophoblast cells,which might be one of the reasons for the occurrence of choriocarcinoma.

关 键 词：AQP8 正常人绒毛滋养细胞 绒癌 

分 类 号：R711.74[医药卫生—妇产科学]