FHIT蛋白和p21蛋白在外阴尖锐湿疣、外阴上皮内瘤变中的表达  

The expression of FHIT protein and p21 protein in vulvar condyloma acuminatum and vulvar intraepithelial neoplasia

作　　者：范余娟[1] 罗雪慧[1] 杨开选[2] 徐红[1] 李雷[2] 刘辉[2] 

机构地区：[1]广西医科大学附一院妇产科,南宁530021 [2]四川大学华西第二医院病理科,成都610041

出　　处：《重庆医科大学学报》2010年第4期527-530,共4页Journal of Chongqing Medical University

基　　金：广西自然科学基金资助(编号:桂科自0640106)

摘　　要：目的：探讨FHIT和p21蛋白在外阴尖锐湿疣（Vulvar condyloma acuminatum,VCA）、外阴上皮内瘤变（Vulvar intraepi thelial neoplasia,VIN）及正常外阴组织中的表达情况。方法：采用免疫组化（SP）法检测51例VCA,39例VIN及20例正常外阴组织中的脆性组氨酸三联体基因（Fagile histidine triad,FHIT）,p21的表达。结果：FHIT在VCA组和VINI-Ⅱ,VINⅢ中的阳性率明显低于正常组（P=0.0001）;FHIT在VCA组和VINⅢ组阳性率无统计学意义（P=0.592）;p21在正常外阴、VIN和VCA组中阳性率差异有统计学意义（P=0.0001）;p21在正常外阴、VIN阳性率无统计学意义（P=0.793）,而在VIN和VCA中差异有统计学意义（P=0.0001）。结论：（1）FHIT和p21基因异常表达与VCA和VIN的发生发展有关。（2）FHIT基因异常是外阴癌变过程中的早期事件。Objective：To probe the expression of Fragile histidine triad（FHIT）and p21 protein in Vulvar Condyloma acuminatum（VCA） and vulvar intraepithelial neoplasia（VIN）.Methods：Immunohistochemistry was used to detect FHIT and p21 expression in 51 cases of VCA,39 cases of VIN and 20 cases of normal vulvar epithelium.Results：The positive rate of FHIT in VCA and VINⅠ～Ⅱ、VINⅢwas obviously lower than which in normal tissue（P=0.000 1）;there was no statistic significance in the positive rate of FHIT expression between VINⅢand VCA（P=0.592）;the positive rate of p21 in normal vulva,VIN and VCA has statistic significance;there was no statistic significance in the positive rate of p21 expression between VIN and VCA（P=0.793）.Conclusion：（1）FHIT and p21 expression are associated with the development and progression of VCA and VIN（.2）The abnormality of FHIT gene is a early event in the development and progression of vulvar cancer.

关 键 词：外阴尖锐湿疣 外阴上皮内瘤变 FHIT P21 

分 类 号：Q786[生物学—分子生物学] Q711.72