间充质干细胞移植对兔颈动脉损伤后的内皮修复作用研究  

Effect of transplantation of mesenchymal stem cells on endothelium repair in rabbits with balloon-induced injury carotid

作　　者：许官学[1] 赵然尊[1] 石蓓[1] 郭艳[1] 王冬梅[1] 沈长银[1] 

机构地区：[1]遵义医学院附属医院心内科,贵州563003

出　　处：《重庆医科大学学报》2010年第4期530-534,共5页Journal of Chongqing Medical University

基　　金：贵州省教育厅重点项目(编号:黔教科2007-040号)

摘　　要：目的:探讨经骨髓动员后自外周血分离培养的间充质干细胞(Mesenchymal stem cells,MSCs)移植对模型兔颈动脉损伤后的修复作用及可能机制。方法:建立兔动脉粥样硬化狭窄模型,粒细胞集落刺激因子(Granulocyte colony-stimulating factor,G-CSF)动员5d后采集外周血,用密度梯度离心法结合反复贴壁法培养获得纯化的MSCs,增强型绿色荧光蛋白(Enhance dgreen fluorescent protein,EGFP)报告基因标记。对模型兔颈动脉进行球囊损伤,术毕立即耳缘静脉内分别注入标记的MSCs(移植组)或培养液(对照组),分别于术后7、14、28d处死动物,取颈动脉血管标本进行形态学和内皮化程度分析,并对归巢的MSCs进行鉴定和分化跟踪。结果:各个时段血管形态学分析均显示移植组内膜增殖程度显著低于对照组(P<0.01);内皮化程度分析显示移植组明显优于对照组(P<0.01)。MSCs移植组于各个时间段均可见血管组织中有EGFP(+)细胞分布,且部分细胞同时为EGFP和CD31阳性,对照组则无。结论:静脉移植MSCs能促进模型兔颈动脉球囊损伤血管的内皮修复,并抑制内膜增生。Objective：To investigate the effect and its possible mechanism of peripheral blood mesenchymal stem cells（MSCs）on endothelium repair in Rabbits with balloon-induced injury carotid.Methods：Purified MSCs were Obtained from peripheral blood through combining density gradient centrifugation with repeating adherence cultivation after the application of Granulocyte colony-stimulating factor（G-CSF）for 5 days in rabbits with atherosclerosis stenosis carotid artery,and marked with enhanced green fluorescent protein（EGFP）.EGFP marked MSCs were transplanted into the rabbits in the transplantation of MSCs groups and equivalence culture solution was injected in the control groups through surrounding ears veins after balloon-induced carotid artery in rabbits.The specimens of carotid artery were harvested to use for the analysis of vessels morphology and endothelialization in 7,14,28 days of post-operative,and the homing MSCs were identified.Results：Carotid artery morphology analysis in each stage indicates that intima hyperplasia is obviously less in the transplantation of MSCs groups than that in the control groups（P〈0.01）.Endothelialization analysis shows that the degree of endothelialization is better in the transplantation of MSCs groups than that in the control groups（P〈0.01）.EGFP-positive cells and EGFP-and CD31-positive cells were found in the carotid artery of each stage in the transplantation of MSCs groups,but there was nothing in the control groups.Conclusion：Intravenous transplantation of PBMSCs may accelerate reendothelialization and attenuate neointimal thickening in rabbits with balloon-induced injury carotid.

关 键 词：间充质干细胞 内皮 颈动脉 兔 

分 类 号：R543.5[医药卫生—心血管疾病]