抗肝纤维化姜黄素脂质体的制备及初步稳定性考察  

Preparation of curcumin liposome and preliminary study on its stability

作　　者：赵怡[1] 孟路华[1] 潘黎军[1] 王驰[1] 

机构地区：[1]重庆医科大学药学院药物化学教研室,重庆400016

出　　处：《重庆医科大学学报》2010年第4期582-585,共4页Journal of Chongqing Medical University

摘　　要：目的:制备姜黄素(Curcumin,Cur)脂质体并考察其包封率及稳定性。方法:采用逆相蒸发法制备Cur脂质体,葡聚糖凝胶柱层析法分离含药脂质体与游离药物并测定包封率;正交设计优选制备处方;离心加速实验与渗漏率的测定考察脂质体的稳定性。结果:所得优化处方为磷脂与药物的重量比60:1,磷脂与胆固醇的重量比4:1,pH6.5的磷酸盐缓冲液水合递质,超声时间为5min。以优化处方制得的脂质体平均包封率达95.06%。结论:选用逆相蒸发法优化Cur脂质体处方合理,工艺简便可行,包封率高,稳定性好。Objective：To prepare curcumin liposome and investigate its stability.Methods：Curcumin liposome was prepared by the reversed-phase evaporation method and purified by exclusion chromatography with Sephadex-50.The optimum formation was selected by means of orthogonal design of experiment;Centrifugal acceleration experiment and determination of leakling ration were served to proved the liposome stability.Results：The optimum formula was as follows：the ratio of lecithin to drug was 60∶1;lecithin：cholesterol was 4∶1;pH of PBS was 6.5,ultrasonic time was 5min.The average entrapment efficiency for curcumin was 95.06%.Conclusion：The selected formulation and preparation technique of curcumin liposome is reasonable in prescription,practicable in techniques,high in encapsulation efficiency and good in stability.

关 键 词：姜黄素脂质体 逆相蒸发法 正交设计 包封率 稳定性 

分 类 号：R931[医药卫生—生药学]