IL-2靶向脂质体的处方筛选及药物含量的测定  

Selection of formulation for IL-2 targeting liposome and determination of drug contents

作　　者：吴镁春[1] 朱莉[1] 王驰[1] 

机构地区：[1]重庆医科大学药学院药物化学教研室,重庆400016

出　　处：《重庆医科大学学报》2010年第4期591-593,共3页Journal of Chongqing Medical University

摘　　要：目的：筛选IL-2靶向脂质体的最佳处方,并对5-氟尿苷（5-fluorouridine,5-FUR）前药进行含量测定。方法：应用正交实验设计选择最佳处方配比;采用紫外分光光度法测定5-FUR前药的含量,检测波长为267nm。结果：筛选最佳处方：药脂比为1：10、磷脂与胆固醇的比例为3：1。5-FUP前药对照品浓度在0.002-0.02mg/ml的范围内与吸收度线性关系良好,回归方程为y=16.357x＋0.007,r=0.9997（n=3）,平均加样回收率为97.09%,RSD=2.05%（n=3）。样品中5-FUR前药含量为18.84-18.91μg/ml。结论：该处方设计合理,紫外分光光度法操作简便、稳定性好、结果可靠,可作为IL-2靶向脂质体中5-FUR前药含量测定方法。Objective：Selection ofthe optimal formulation for IL-2 targeting liposome and determination ofcontent for 5-FUR.Methods：orthogonal test method was used to selection the optimal formulation,UV spectrophotometry was used to determine the 5-FUR prodrug content,and the detection wavelength was at 267 nm.Results：The optimal formulation showed Drug/EPC 1∶10,EPC/Ch 3∶1.The absorbency and concentration of 5-FUR prodrug had good linearity in the range of 2.0～20.0μg/mL.The regression equation was y=16.357x＋0.007,r= 0.999 7（n=3）,The 5-FUR prodrug content was 18.84～18.91μg/ml.The average recovery rate was 97.09%,and RSD=2.05%（n=3）.Conclusion：Formulation was designed reasonablely.The method is simple,stable and reliable,which can be used to determine the 5-FUR prodrug in IL-2 targeting liposome.

关 键 词：5-FUR前药 正交设计 紫外分光光度法 含量测定 

分 类 号：R931[医药卫生—生药学]