葡萄糖胺抑制LL-37诱导的人脐带血管内皮细胞活化  

Glucosamine inhibits activation of HUVEC induced by LL-37

作　　者：具英花[1] 于爱鸣[1] 解智慧[1] 孙秀华[1] 

机构地区：[1]中国医科大学生物化学与分子生物学教研室,辽宁沈阳110001

出　　处：《解剖科学进展》2010年第3期226-229,共4页Progress of Anatomical Sciences

基　　金：辽宁省科技厅博士科研启动基金资助项目(20091105)

摘　　要：目的探讨葡萄糖胺对LL-37诱导的人脐带血管内皮细胞(HUVEC)细胞间黏附分子-1(intercellular adhesion molecule,ICAM-1)和单核细胞趋化因子(monocyte chemoattractant factor,MCP-1)表达的影响。方法实时逆转录聚合酶链式反应(Rea ltime RT-PCR)和免疫印迹法(Western blot)、酶联免疫吸附测定(enzyme linked immunosorbent assay)分别测定ICAM-1和MCP-1的表达;Western blot法测定HUVEC蛋白的O-连接N-乙酰葡萄糖胺(O-GlcNAc)修饰及细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)的磷酸化程度。结果葡萄糖胺抑制LL-37诱导的ICAM-1和MCP-1在HUVEC的表达;葡萄糖胺诱导内皮细胞蛋白的O-GlcNAc修饰;葡萄糖胺抑制LL-37诱导的ERK磷酸化。结论葡萄糖胺通过抑制ERK的活化抑制LL-37诱导的ICAM-1和MCP-1的表达。Objective To study the effect of glucosamine on the expression of intercellular adhesion molecule-1 （ICAM-1） and monocyte chemoattractant factor-1 （MCP-1） induced by cathelicidin LL-37 in human umbilical vein endothelial cell （HUVEC）. Methods Real time RT-PCR, Western blot and enzyme linked immunosorbent assay （ELISA） were used for detection of ICAM-1 and MCP-1 expression. Western blot was used to detect O-linked-N-acetyl glucosamine （O-GlcNAc） modification and extracellular signal-regulated kinase （ERK） modification in HUVEC. Results Glucosamine inhibited ICAM-1 and MCP-1 expression and ERK phosphorylation induced by LL-37 and induced protein O-GlcNAc modification in HUVEC. Conclusion Inhibition of glucosamine on ICAM-1 and MCP-1 expression might be related to ERK suppression in HUVEC.

关 键 词：葡萄糖胺 细胞间黏附分子-1 单核细胞趋化因子-1 脐带血管内皮细胞 人 

分 类 号：R331.3[医药卫生—人体生理学]