雷公藤甲素对大鼠垂体促肾上腺皮质激素含量的影响  

Effect of Triptolide on Content of Adreno-Corticotropic Hormone in Pituitary of Rats

作　　者：张武[1] 朱建华[1] 关伟[1] 

机构地区：[1]皖南医学院

出　　处：《医学研究杂志》2010年第5期54-56,F0003,共4页Journal of Medical Research

基　　金：安徽省教育厅自然科学研究项目(2006KJ413B)

摘　　要：目的了解较长期使用雷公藤甲素对正常大鼠血浆促肾上腺皮质激素(ACTH)含量的影响。方法雄性SD大鼠20只随机分为2组,连续灌胃7周,其中空白对照组10只:2%丙二醇2ml/kg,bid;雷公藤甲素组10只:2%丙二醇雷公藤甲素混溶液20μg/kg,bid。用放射免疫分析法检测两组大鼠血浆促肾上腺皮质激素(ACTH)含量,用免疫细胞化学染色法观察ACTH在两组大鼠垂体中的表达。结果雷公藤甲素组血浆ACTH含量与空白对照组有显著差异;ACTH在雷公藤甲素组大鼠垂体中的表达高于空白对照组。结论雷公藤甲素对下丘脑-垂体-肾上腺轴(HPAA)可能作用机制之一是兴奋垂体ACTH神经细胞,使垂体分泌、释放ACTH增加。Objective To Study the effect of long - term triptolide use on the content of the normal rat plasma ACTH. Methods 20 male SD rats were divided into 2 groups randomly, and gavaged for 7 weeks . The control group was 10 with 2% propylene glycol 2ml/kg, bid ; the group of Triptolide was 10 with 2% the mixture of propylene glycol and Triptolide 20μg/kg, bid. We used radioimmunoassay to detecet the content of ACTH in rat＇s plasma and immunocytochemistry to detecet the expression of ACTH in pituitary. Results The content of ACTH in the group of the Triptolide had significant difference as compared with control group. The expression of ACTH in the group of the Triptolide was higher than that of control group. Conclusion These results suggested that one of the possible mechanism of the Triptolide in the HPAA might be exciting pituitary ACTH neurons,thus causing the content of ACTH increasing.

关 键 词：雷公藤甲素 大鼠 血浆促肾上腺皮质激素 

分 类 号：R285.5[医药卫生—中药学]