苜草素多糖、黄酮和皂苷对小鼠巨噬细胞RAW264.7β-防御素基因表达的影响  

Effect of Polysaccharide,Flavonoids and Saponins of Polysavone on Expression of Mouse Beta-defensins in RAW264.7 Cells

作　　者：杨江涛[1] 董晓芳[1] 佟建明[1] 刘晴雪[1] 张琪[1] 吴莹莹[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193

出　　处：《畜牧兽医学报》2010年第5期608-614,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："十一五"国家科技支撑计划项目(2006BAD12B05);农业科技成果转化资金项目(2007GB23260400);中央级公益性科研院所基本科研专项资金(ywf-td-4)

摘　　要：本研究旨在观察苜草素活性成分多糖、黄酮、皂苷对小鼠β-防御素表达的影响。分别采用含有30、60、90、150μg.mL-1苜草素多糖、含有10、20、30、50μg.mL-1苜草素黄酮、含有30、60、90、150μg.mL-1苜草素皂苷的细胞培养液处理RAW264.7细胞,观察其对小鼠RAW264.7细胞β-防御素(mouse beta-defensin,mBD)mRNA表达水平的影响。结果发现,苜草素多糖对mBD1的表达水平没有影响,对mBD2、mBD3的表达有促进作用,30μg.mL-1效果最明显;苜草素黄酮促进mBD1的表达,10μg.mL-1效果最明显,对mBD2、mBD3的表达有抑制作用;苜草素皂苷对mBD1的表达有促进作用,对mBD2的表达水平没有影响,对mBD3表达有抑制作用。苜草素中的多糖、黄酮、皂苷对RAW264.7细胞mBDs表达的作用效果不同,苜草素多糖可能主要促进动物的特异性免疫功能,苜草素黄酮、皂苷可能促进动物的先天性免疫功能。To investigate the effect of polysaccharide,flavonoids and saponins of Polysavone on the expression of mouse beta-defensins in RAW264.7 cells,RAW264.7 cells were cultured with Polysavone polysaccharide at 30,60,90,150 μg·mL-1,Polysavone flavonoids at 10,20,30,50 μg·mL-1,Polysavone saponins at 10,20,30,50 μg·mL-1,respectively.The mRNA expression of mouse beta-defensin（mBD1-3） were detected.The results showed that the mBD2 and mBD3 mRNA expression could be improved by Polysavone polysaccharide,especially the treatment with 30 μg·mL-1,but there was no effect on the mRNA expression of mBD1.The mBD1 mRNA expression could be improved by Polysavone flavonoids,especially the treatment with 10 μg·mL-1,the mBD2 and mBD3 mRNA expression could be inhibited.The mBD1 mRNA expression could be improved by Polysavone saponins,the mBD3 mRNA expression could be inhibited,but Polysavone saponins had no effects on the mRNA expression of mBD2.The results showed the different effects of polysaccharide,flavonoids and saponins of Polysavone on the mRNA expression of mBD1,mBD2 and mBD3.The Polysavone polysaccharide may enhance the function of adaptive immunity.The flavonoids and saponins of Polysavone may enhance the function of innate immunity.

关 键 词：Β-防御素 苜草素 多糖 黄酮 皂苷 

分 类 号：S816.79[农业科学—饲料科学] Q344.13[农业科学—畜牧学]