产苦马豆素真菌的筛选与鉴定  

Screening and Identification of Swainsonine-Secreting Fungal

作　　者：马尧[1] 路浩[1] 赵宝玉[1] 来航线[1] 李蓉[1] 王占新[1] 陈基萍[1] 卢围[1] 荣杰[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2010年第5期621-629,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：西北农林科技大学动物医学院院长基金项目

摘　　要：为探明青海及内蒙古多种疯草类植物及其根际土壤中是否存在产生苦马豆素的真菌,并对其种属进行鉴定,同时测定其苦马豆素产率。本试验拟对采自内蒙古和青海的疯草样品中的内生真菌及根际土壤真菌进行分离;应用薄层色谱和气相色谱法分别检测菌丝及发酵液中苦马豆素含量,筛选可产生苦马豆素的真菌;运用形态学和分子生物学方法对其属种进行鉴定。从青海采集的疯草样品中分离并筛选出2株可产苦马豆素真菌,一株为土壤真菌,一株为内生真菌,分别命名为FS-5和EFG-7;菌丝中苦马豆素含量分别为0.773和0.11 mg.g-1,结合形态学和分子生物学试验结果,确定这2株真菌分别为裂褶菌属真菌和镰孢菌(霉)属三线镰刀菌。结果显示,我国青海疯草及根际土壤中存在可产生苦马豆素的真菌。The objective of this study was to explore whether the fungus of swainsonine-secret exist in locoweeds and rhizosphere soil,identify genus of those fungus and determinate their swainsonine yield in Qinghai Province and Inner Mongolia Autonomous Region.Fungus was isolated from the locoweed and soil samples,and swainsonine was detected by thin layer chromatography and gas chromatography for isolated fungus.Two strains of endophytic fungi produced the alkaloid swainsonine were isolated and obtained from locoweeds and rhizosphere soil,and named FS-5 and EFG-7,and SW yield is 0.773 and 0.11 mg·g-1,respectively.Morphological evidence and sequence analysis of the ITS region suggest that FS-5 is most closely related to Schizophyllum sp,and EFG-7 is Fusarium tricinctum.The results showed that the fungus of swainsonine-producing exist in locoweeds and rhizosphere soil in Qinghai Province.

关 键 词：苦马豆素 内生真菌 裂褶菌属 三线镰刀菌 疯草 

分 类 号：S852.66[农业科学—基础兽医学]