不同地区海兰褐蛋鸡中J亚群-禽白血病病毒株gp85基因的分子演化分析  

Molecular Evolution Analysis for gp85 Gene of Subgroup J-Avian Leukosis Viruses Strains from Hyline Egg-type Chickens in Different Areas

作　　者：代阳[1] 杨其峰[1] 王波[1] 刘绍琼[1] 王秀臻[1] 柴家前[1] 崔治中[1] 孙淑红[1] 

机构地区：[1]山东农业大学动物医学院,泰安271018

出　　处：《畜牧兽医学报》2010年第5期635-638,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家公益性行业(农业)科研专项(200803019);山东省中青年科学家基金(2008BS07004);山东农业大学青年科技创新基金

摘　　要：本研究通过对不同地区海兰褐蛋鸡群中分离的5株J亚群-禽白血病病毒(ALV-J)的囊膜糖蛋白基因(gp85)进行同源性分析,阐述了不同海兰褐鸡群中存在的ALV-J的分子演化规律。对2008-2009年分别从北京、陕西、山东泰安、济阳、曲阜等不同地区饲养的海兰褐鸡分离到的5株ALV-J,用PCR方法克隆gp85基因、测序,并与国内外已发表的14株ALV-Jgp85基因进行同源性比较。结果表明,5株ALV-J与来自白羽肉鸡的HPRS-103株的同源性最近,平均为96.6%(96.4%～96.8%);与来自国内海兰灰蛋鸡的SD07LK1株的同源性平均仅为89.6%(89.3%～89.9%);而5株ALV-J间的同源性高达98.1%以上(98.1%～100%)。本研究发现,不同地区的海兰褐蛋鸡中广泛存在的ALV-J可能有一个共同的来源,即国外的白羽肉鸡。By the homology analysis for gp85 genes of 5 strains of ALV-J isolated from Hyline laying hens,the molecular evolution of ALV-J was studied in the present study.The 5 strains of ALV-J were obtained from Beijing,Shanxi and Shandong.The gp85 genes were cloned by PCR and the sequence was measured and compared with 14 published reference ALV-J strains.The homology analysis showed that the 5 isolated strains of ALV-J shared 96.6%（96.4%-96.8%） and 89.6%（89.3%-89.9%） homology with the HPRS-103 strain isolated from white meat-type broiler and SD07LK1 strain isolated from egg-type chickens.The homology of the 5 strains was exceeded 98.1%（98.1%-100%）.The result indicated that ALV-J strains from Hyline egg-type chickens may have the same origin,the white meat-type broiler chickens imported abroad.

关 键 词：海兰褐蛋鸡 J亚群-禽白血病病毒 GP85基因 同源性 

分 类 号：S852.659.3[农业科学—基础兽医学]