镍钛合金在豚鼠听泡的生物相容性观察  

The Biocompatibility of Nitinol in Guinea pigs’ Acoustic Vesicle

作　　者：张媛媛[1] 肖自安[1] 李周[2] 徐国富[2] 黄伯云[3] 

机构地区：[1]中南大学湘雅二医院耳鼻咽喉-头颈外科,长沙410011 [2]中南大学材料科学与工程学院 [3]中南大学粉末冶金研究院

出　　处：《听力学及言语疾病杂志》2010年第3期211-214,共4页Journal of Audiology and Speech Pathology

基　　金：中南大学博士后基金(56890)资助

摘　　要：目的探讨镍钛形状记忆合金(nitinol,NiTi)植入体在豚鼠听泡的生物相容性。方法健康、听觉灵敏的纯白红目豚鼠50只,一耳为实验组(NiTi植入组,50耳),其中25只对侧耳为对照组I[纯钛(Ti)植入组],另25只对侧耳为对照组II(空白组)。分别于术后7、14、28、56、112d随机处死含对照组I和对照组II的豚鼠各5只,观察听泡内有无肉芽生长等炎性反应、材料外观有无蚀斑和颜色改变,以Sirion200场发射扫描电镜(FEI公司,美国)观察材料表面新生组织,GENESIS60SEDAX能谱仪(EDAX公司,美国)能谱分析新生组织的元素构成。结果 NiTi及Ti植入体表面均有新生骨骼样组织生长,有纤维状物与听泡壁及听小骨相连,周围无明显肉芽组织,植入材料表面无蚀斑和颜色改变,有新生骨骼样组织形成,从术后7d的零星点状或条索状逐渐增多交织成网状到112天时形成板状,能谱分析证实为骨组织,但NiTi植入组新生组织中含有极微量的镍(Ni)。结论 NiTi在豚鼠听泡有很好的生物相容性,但有微量的Ni释放,其生物毒性需进一步观察。Objective To explore the biocompatibility of Nitinol（NiTi） after being implanted in guinea pigs＇ acoustic vesicle. Methods 50 healthy guinea pigs with normal hearing were used in this study. One ear of each guinea pig was selected as the experimental group randomly （NiTi implanted in the acoustic vesicle, 50 ears）. The lefte ears of the guinea pigs were divided into control group Ⅰ （Ti implanted group, 25 ears） and control group Ⅱ （opened acoustic vesicle without implanting, 25 ears） equally. 5 guinea pigs in control Ⅰ and 5 guinea pigs in control Ⅱ were executed at 7th, 14th, 28th, 56th, ll2th day after operation, respectively. Whether there was granulation hyperplasia around the implanted materials, and corrosion spot and colour changing occurred on the surface of the implanted materials were observed. Then, the surface topography and the elemental composition of the neonatal tissues on the surface of the materials were observed and analyzed by the Field Emission Scanning Electron Microscope （FEI Co, USA） and the Energy Dispersive Analysis （EDAX Co, USA）, respectively. Results There was neonatal bone--like tissue on the surfaces of NiTi implant but no inflammatory granulation tissue around them, and no corrosion spot and colour changing occurred on the surface of the implanted NiTi and in the Ti implant. Neonatal tissue scatterred on the surface of NiTi and Ti at 7th day, gradually interweaved into mesh from 14th to 56th day and finally developed to plate at 112th day after operation. The neonatal bone on NiTi contained very small amount of Ni and Ti elements and that on Ti implant with very slight of Ti element. Conclusion The bone--formation reaction and biocompatibility of NiTi in the acoustic vesicle are outstanding, but with very small amount of Ni releasing from NiTi and its biological toxicity should be further studied.

关 键 词：镍钛形状记忆合金 生物相容性 豚鼠 听泡 

分 类 号：R764.92[医药卫生—耳鼻咽喉科]