缺氧相关因子HIF-1α、AQP-1表达与宫颈鳞状细胞癌血管生成关系的研究  

Study of relationship between expressions of HIF-1α,AQP-1 and angiogenesis in cervical squamous cell carcinoma

作　　者：舒丽莎[1] 张凡[2] 陈江平[1] 常永霞[3] 张九鸿[2] 赵秀芳[2] 成日青[2] 

机构地区：[1]河北北方学院附属第一医院妇产科,张家口075000 [2]河北北方学院附属第一医院病理科,张家口075000 [3]河北北方学院附属第一医院康复医学中心,张家口075000

出　　处：《现代妇产科进展》2010年第4期249-252,共4页Progress in Obstetrics and Gynecology

基　　金：河北省科技厅科技攻关项目(No:072761585);张家口市科学与发展计划基金资助(No:0711045D-6;0911019D-9)

摘　　要：目的:探讨HIF-1α、AQP-1表达对宫颈鳞状细胞癌血管生成的作用。方法:应用免疫组织化学方法检测54例宫颈鳞状细胞癌中HIF-1α、AQP-1的表达,用CD105标记肿瘤微血管密度。结果:HIF-1α在宫颈鳞状细胞癌的阳性表达率为50%,表达水平与分化程度、浸润深度明显相关(r=-0.674,P<0.05和r=0.897,P<0.01),与临床分期、肿瘤体积、淋巴结转移无明显相关(P>0.05)。AQP-1在宫颈鳞状细胞癌的表达率为87.1%,表达水平与肿瘤体积、临床分期明显相关(r=0.989,P<0.05和r=0.807,P<0.01),与分化程度、淋巴结转移、浸润深度无明显相关(P>0.05)。微血管密度与肿瘤体积、浸润深度明显相关(r=0.609,P<0.05和r=0.845,P<0.01);与临床分期、分化程度、淋巴结转移无明显相关(P>0.05)。微血管密度与HIF-1α表达明显相关(r=0.701,P<0.05),而与AQP-1的表达无关(P>0.05)。AQP-1的表达水平与HIF-1α的表达水平明显相关(r=0.648,P<0.05)。结论:HIF-1α、AQP-1均参与宫颈鳞状细胞癌缺氧诱导新生血管过程,并且与微血管密度密切相关,其中HIF-1α发挥重要作用。Objective：To explore the effection of HIF-1α,AQP-1 on angiogenesis in cervical squamous cell carcinoma.Methods：The expressions of proteins HIF-1α,AQP-1 were detected by immmunohistochemistry method in 54 cases of cervical squamous cell carcinoma.The micro vessel density（MVD） were detected by CD105.Results：The positive expression ratio of HIF-1α in cervical squamous cell carcinoma was 50%.Expression of HIF-1α related to tumor differentiation,invasive depth（r=-0.674,P〈0.05;r=0.897,P〈0.001）,but not to tumor volume,clinical stage,lymphonode metastasis（P〉0.05）.The positive expression ratio of AQP-1 was 87.1%.The expression of AQP-1 had relationship with tumor volume,clinical stage（r=0.989,P〈0.05;r=0.807,P〈0.001）,but not with the tumor differentiation,invasive depth and lymphonode metastasis（P〉0.05）.There was relationship between the expression of HIF-1α and tumor microvessel density（r=0.701,P〈0.05）.There was no relationship between the expression of AQP-1 and tumor microvessel density（P〈0.05）.The tumor microvessel density had relationship with tumor volume,invasive depth（r=0.609,P〈0.05;r=0.845,P〈0.001）,but had no relationship with tumor differentiation,clinical stage,lymphonode metastasis（P〈0.05）.There was relationship between the expression of HIF-1α and AQP-1（r=0.648,P〈0.05）.Conclution：HIF-1α,AQP-1 both take part in the procession of angiogenesis induced by hyposia in cervical squamous cell carcinoma and have relationship with tumor microvessel density;HIF-1α plays key role in the whole procession.

关 键 词：宫颈鳞癌 水孔蛋白类 缺氧诱导因子-1 血管生成 

分 类 号：R711.7[医药卫生—妇产科学]