小鼠骨髓间充质干细胞向子宫内膜上皮细胞方向分化的体外实验  

Differentiation of mice's marrow mesenchymal stem cells into endometrial epithelial cells in vitro

作　　者：张雯碧[1] 程明军[1] 徐丛剑[1] 

机构地区：[1]复旦大学附属妇产科医院,上海200011

出　　处：《现代妇产科进展》2010年第4期257-260,共4页Progress in Obstetrics and Gynecology

基　　金：2008年国家自然科学基金资助(No:30772310)

摘　　要：目的:探讨骨髓间充质干细胞(BMSCs)体外向子宫内膜上皮细胞方向分化的可行性。方法:原代培养小鼠BMSCs和子宫内膜间质细胞(ESCs)并鉴定,将BMSCs与ESCs共培养,并补充生长因子(TGF-β10ng/ml,EGF10ng/ml,PDGF-BB10ng/ml)和雌激素(17-β-雌二醇1×10-7mol/L),应用细胞免疫荧光染色法检测分化的BMSCs是否表达上皮细胞特异标记角蛋白。结果:小鼠BMSCs增殖潜能强,呈克隆性生长,其表面标记Sca-1,CD117,CD29,CD34表达率分别为5.36%,2.41%,2.63%,0.68%。小鼠ESCs特异标记波形蛋白表达阳性,角蛋白阴性。小鼠BMSCs与ESCs共培养5天后大部分细胞形态由成纤维样向上皮样细胞变化,细胞免疫荧光染色方法鉴定诱导分化后的BMSCs表达角蛋白。结论:小鼠BMSCs在ESC分泌和外源性因素诱导下可向子宫内膜上皮细胞方向分化。生长因子和雌激素在此过程中起重要作用。Objective：To explore the plasticity of differentiation of bone mesenchymal stem cells（BMSCs） into endometrial epithelial cells in vitro.Methods：Mice＇s BMSCs and endometrial stromal cells（ESCs） were isolated and identified in certain ways.The BMSCs were induced by co-cultured with ESCs,added 10ng/ml TGF-β,10ng/ml EGF,10ng/ml PDGF-BB,1×10-7mol/L β-estradiol.The expression of cytokeratin which was sign of endometrial epithelial cells was tested by immunofluorescence in differentiated BMSCs.Results：BMSCs in vitro showed great potential of proliferation.The superficial markers of BMSCs：Sca-1,CD117,CD29,CD34 were 5.36%,2.41%,2.63%,0.68% respectively,indicating the cultured cells were BMSCs.After co-cultured with ESCs for five days,most differentiated BMSCs looked like epithelial cells.The differentiated BMSCs expressed cytokeratin,indicating that BMSCs had been differentiated into endometrial epithelial cells to some extent.Conclusion：BMSCs has the potential to differentiate into endometrial epithelial cells in vitro.The exogenous growth factors and estradiol play an important role.

关 键 词：骨髓间充质干细胞 子宫内膜异位症 分化 小鼠 近交C57BL 

分 类 号：R711.71[医药卫生—妇产科学]