缬草对慢性应激导致的抑郁大鼠大脑海马p-CREB阳性神经元数量的影响  

Effects of Valerian on number of p-CREB positive neurons in cerebral hippocampus of rats with depression induced by chronic mild stress

作　　者：周春春[1] 曾园山[1] 秦亚静[1] 丁英[1] 李燕[1] 钟志强[1] 

机构地区：[1]中山大学中山医学院组织胚胎学教研室,广东广州510080

出　　处：《解剖学研究》2010年第2期81-87,共7页Anatomy Research

摘　　要：目的探讨最佳剂量缬草对慢性应激导致的抑郁大鼠行为及其大脑海马磷酸化cAMP反应元件结合蛋白(phosphorylated cAMP responsive element-binding protein,p-CREB))阳性神经元的影响。方法将35只大鼠随机均分为正常对照组、未用药模型组、阴性对照模型组、阳性对照模型组、低剂量缬草模型组、中剂量缬草模型组和高剂量缬草模型组。在用药期间,每周测试大鼠体质量、自来水及1%糖水摄取量1次。用药后,计数大脑海马神经元数量,确定缬草最佳剂量。然后根据前述方法,在灌药结束后灌注和固定所有大鼠。采用中性红染色、免疫组化等方法对大鼠海马p-CREB和脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)阳性神经元进行检测。结果高剂量(100mg·kg-1·d-1)缬草能促使抑郁大鼠1%糖水摄取量恢复至正常水平,还可使抑郁大鼠大脑海马神经元以及p-CREB阳性神经元数量恢复到正常水平。正常对照组大鼠大脑海马神经元的BDNF染色较浅淡,其余各组大鼠大脑海马神经元的BDNF阳性染色没有明显差异。结论本研究高剂量(100mg·kg-1·d-1)缬草可能是治疗慢性应激导致大鼠抑郁症的最佳剂量。缬草可以改善抑郁大鼠的行为活动,恢复大脑海马神经元以及p-CREB阳性神经元数量到正常水平。Objective To explore the effects of the optimal dosage Valerian on behavior, and phosphorylated cAMP responsive element-binding protein （p-CREB） positive neurons in cerebral hippocampus of rats with depression induced by chronic mild stress. Methods Thirty five rats were randomly divided into 7 groups： normal control group, untreated group, negative control group, positive control group, and low-, medium- and high-dose Valerian-treated groups. During the intragastric administration, the body weight, tap water intake and 1% sucrose intake were examined to all groups of rats once a week. After the administration, the neuronal number of cerebral hippocampus was counted, the optimal dosage Valerian was obtained eventually. According to the above methods, all rats were perfused and fixed after the intragastric administration was over. The p- CREB and brain-derived neurotrophic factor （BDNF） positive neurons of hippocampal sections were detected by immunohistochemistry and neutral red staining. Results 1% sucrose intake of rats with depression recovered to the normal level after the optimal dosage Valerian was administered, and the number of neurons and p-CREB positive neurons in cerebral hippocampus of rats with depression were also increased to the normal level. BDNF positive neurons of hippocampus in the Normal control group were lightly stained. There was no obvious difference in BDNF positive neuron staining of cerebral hippocampus in the rest groups of rats compared with the Normal control group. Conclusion High-dose （100 mg. kg-1. d-1） Valerian may be a optimal dosage to treat the rats with depression induced by chronic mild stress in the present study. Valerian may improve the behavior activity and recover the number of neurons and p-CREB positive neurons of cerebral hippocampus in the depressive rats to the normal level.

关 键 词：缬草 应激 抑郁症 海马 磷酸化cAMP反应元件结合蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]