流行性乙型脑炎患儿白介素1β、神经元特异性烯醇酶的变化及临床意义  

Clinical research on the changes of interleukin-1β,neuron-specific enolase in children with Japanese encephalitis

作　　者：石海矾[1] 李伟[1] 陈益平[1] 汪洪姣[1] 张桂莲[1] 金胜鑫[2] 

机构地区：[1]温州医学院附属第二医院儿科,浙江温州325027 [2]温州医学院附属第二医院检验科,浙江温州325027

出　　处：《温州医学院学报》2010年第2期149-151,155,共4页Journal of Wenzhou Medical College

摘　　要：目的:研究流行性乙型脑炎(乙脑)患儿脑脊液、血清白介素1β(IL-1β)与神经元特异性烯醇酶(NSE)的变化及其临床意义。方法:50例乙脑患儿为观察组,按Glasgow评分分正常组17例,轻度组20例,重度组13例。检测极期、恢复期脑脊液及血清IL-1β、NSE含量,并与20例非中枢神经系统疾病患儿进行对照。IL-1β采用双抗体夹心酶联免疫法检测,NSE采用电化学发光法测定。结果:乙脑患儿脑脊液、血清中IL-1β及NSE含量在极期、恢复期皆比对照组显著增高(P均<0.01),且增高程度与病情严重程度相平行(P<0.01)。脑脊液与血清IL-1β含量及脑脊液与血清NSE含量分别呈正相关(P<0.01),脑脊液IL-1β与NSE含量及血清IL-1β与NSE含量亦分别呈正相关(P<0.01)。结论:脑脊液及血清IL-1β,NSE含量测定对乙脑患儿脑损伤程度及临床病情的判断有重要参考价值。Objective： To study the changes of interleukin-1β（IL-1β） and neuron-specific enolase （NSE） both in cerebrospinal fluid （CSF） and serum in children with Japanese encephalitis and the clinical significance of the changes.Methods： Fifty cases of children with Japanese encephalitis were observed,which were divided into three groups according to Glasgow score： normal group （17 cases）,general group （20 cases） and severe group （13 cases）.IL-1β and NSE were detected both in CSF and serum in the very and recovery period in 50 cases,and at the same time 20 cases of children without central nervous system disease were selected as control.IL-1β was determined with enzymelinked immunosorbent assay （ELISA）,NSE was determined with electrochemiluminescence.Results： The levels of IL-1β and NSE in CSF and serum in children with Japanese encephalitis in very and recovery period were both significantly higher than those of the control （P 0.01）,and the degree was parallel to the severity of the disease （P 0.01）.The levels of IL-1β in CSF and serum were positively correlated （P 0.01）,so were the levels of NSE in CSF and serum （P 0.01）.Simultaneously,the levels of IL-1β and NSE in CSF were positively correlated （P 0.01）,so were the levels in serum （P 0.01）.Conclusion： To detect the IL-1β and NSE in CSF and serum is helpful for our judgement of the degree of children’brain injured and assessment of clinical condition of children with Japanese encephalitis.

关 键 词：白介素-1Β 神经元特异性烯醇酶 流行性乙型脑炎 脑脊液 血清 

分 类 号：R725.1[医药卫生—儿科]