电磁场对大鼠骨髓间充质干细胞成纤维细胞生长因子-2和成纤维细胞生长因子受体-2mRNA表达的影响  

The effect of electromagnetic field stimulation on the mRNA expression of fibroblast growth factor and it＇s receptor in bone marrow mesenchymal stem cells in vitro

作　　者：刘朝旭[1] 杨勇[1] 虞冀哲[1] 李贵振[1] 郑亚东[1] 彭飞[1] 赵文春[2] 吴华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科,武汉430030 [2]海军工程大学电力电子技术应用研究所

出　　处：《中华物理医学与康复杂志》2010年第4期253-257,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：国家自然科学基金项目（50477043）

摘　　要：目的 研究电磁场对体外培养的大鼠骨髓间充质干细胞（BMSCs）成纤维细胞生长因子-2（FGF-2）和成纤维细胞生长因子受体-2（FGFR-2）mRNA表达的影响.方法 体外分离培养大鼠骨髓间充质干细胞,取第3代细胞分组经电磁场暴磁处理,然后采用逆转录-聚合酶链反应（RT-PCR）技术分别检测各组细胞的FGF-2和FGFR-2 mRNA表达,并进行定量分析.结果 适当频率及作用时间的电磁场刺激可使大鼠骨髓间充质干细胞FGF-2和FGFR-2 mRNA表达明显增强.用15 Hz、1.0 mT电磁场刺激BMSCs.FGF-2mRNA的表达于10 min时达最大值,而FGFR-2 mRNA的表达则于30 min时达最大值;在50 Hz、1.0 mT电磁场的刺激下,FGF-2 mRNA的表达于60 min时达最大值,而FGFR-2 mRNA的表达则于30 min时达最大值;在75 Hz、1.0 mT电磁场的刺激下,FGF-2和FGFR-2 mRNA的表达均于30 min时达最大值;1.0 mT电磁场刺激30 min条件下,电磁场频率为50 Hz暴磁组BMSCs的FGF-2 mRNA的表达达最大值,而电磁场频率为75 Hz暴磁组BMSCs的FGFR-2 mRNA的表达达最大值.结论 适当＂窗口＂的电磁场刺激对体外培养的大鼠骨髓间充质干细胞FGF-2和FGFR-2 mRNA表达有明显的促进作用.Objective To study the effects of an electromagnetic field （EMF） on the expression of fibroblast growth factor （FGF-2） and it＇s receptor （FGFR-2） mRNA in rat bone marrow mesenchymal stem cells （BMSCs） in vitro. Methods Rat BMSCs were isolated and cultured in vitro. The subcultured cells were divided into different groups to he EMF stimulated at 1.0 roT. The expression of FGF-2 and FGFR-2 mRNA were detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results Different frequencies and durations of 1.0 mT EMF exposure induced FGF-2 and FGFR-2 mRNA expression in comparison to blank controls. The expression of FGF-2 mRNA reached a peak after stimulation at 15 Hz for 10 min, 50 Hz for 60 rain and 75 Hz for 30 min. And the expression of FGFR-2 mRNA reached a peak after 30 minutes at all frequencies. At 1.0 mT with 30 rain exposure, the expression of FGF-2 mRNA peaked after 50 Hz stimulation, and the expression of FGFR-2 mRNA peaked after stimulation at 75 Hz. Conclusions Moderate EMF stimulation can significantly increase the expression of FGF-2 and FGFR-2 mRNA in rat BMSCs in vitro.

关 键 词：骨髓间充质干细胞 成纤维细胞生长因子 电磁场 

分 类 号：R3[医药卫生—基础医学]