氯离子通道阻断剂对大鼠离体海马神经元凋亡的影响  

Study on chloride channel involved in the cultured hippocampal neuronal apoptosis

作　　者：常全忠[1] 张淑玲[1] 蔡仕宁[1] 尹金宝[1] 

机构地区：[1]遵义医学院珠海校区生理学教研室,广东珠海519041

出　　处：《中国老年学杂志》2010年第9期1230-1232,共3页Chinese Journal of Gerontology

基　　金：贵州省科技厅资助项目(No.20072107);珠海市科技局资助项目(PC2008101);遵义医学院博士起动基金(12209)

摘　　要：目的通过观察氯离子(Cl-)通道阻断剂对一氧化氮(NO)供体3-吗啉斯德酮胺(SIN-1)诱导的大鼠离体海马神经元凋亡的效应,探讨Cl-通道在缺血性脑损伤中的作用。方法离体培养12d的SD大鼠海马神经元,随机分为正常对照组、SIN-1处理组、SIN-1处理后和Cl-通道阻断剂组,对各组神经元分别在相应的时间点进行Hoechst荧光染色观察凋亡细胞数和MTT实验定量检测神经元的存活率。结果SIN-1能明显诱导神经元凋亡(P<0.05),SITS和DIDS呈剂量依赖性地抑制NO诱导的神经元损伤,提高神经元的存活率,与SIN-1组相比差异显著(P<0.05)。结论Cl-通道阻断剂对NO诱导的大鼠海马神经元凋亡有一定的保护作用。Objective To observe the effects of two kinds of chloride channel blockers SITS and DIDS on the hippocampal neuronal damage induced by NO in culture and explore the roles of chlorides in the cerebral ischemic injury. Methods The cultures were divided into 3 groups： control,NO treatment,NO treatment plus chloride channel blocker groups. The cultures were detected with the methods of morphological stain （Hoechst 33258） and MTT quantitative analysis to observe the apoptotic neurons and neuronal viabilities. Results There was a significant protective effects of SITS and DIDS on neuronal damage with dose-dependence. Conclusions Chloride channel blockers have some protective effects against neuronal injury induced by NO.

关 键 词：氯离子通道 NO 海马 大鼠 凋亡 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学] R322-81[医药卫生—基础医学]