粗大异体神经两种化学萃取方法的比较  

Comparison of two extraction techniques for larger chemically aceilular nerve allografts

作　　者：王鑫[1] 王玉[1] 彭江[1] 郭全义[1] 张莉[1] 赵斌[1] 许文静[1] 卢世璧[1] 

机构地区：[1]解放军总医院全军骨科研究所、北京市复兴路28号,100853

出　　处：《中国矫形外科杂志》2010年第9期748-751,共4页Orthopedic Journal of China

基　　金：国家自然科学基金项目(编号:30571875);北京市自然科学基金重点项目(编号:03G502)

摘　　要：[目的]采用两种不同的化学法萃取粗大的异体神经,对比观察这两种方法在萃取效果方面的差别。[方法]取新鲜猪坐骨神经,每段长60mm,分别在Triton-100、脱氧胆酸钠及SB-10、SB-16、Triton-200中去细胞处理。实验分两组:Ⅰ组,Triton-100、脱氧胆酸钠去细胞2遍;Ⅱ组SB-10、SB-16、Triton-200去细胞处理2遍。另设对照组:新鲜未处理的猪坐骨神经。从去细胞程度、基底膜板层素活性、脱髓鞘程度及神经纤维管道完整性4个方面进行评价。[结果]所有实验组神经段神经纤维管道尚可,Ⅰ组较Ⅱ组去细胞完全,laminin成分保存;Ⅰ组脱髓鞘完全,Ⅱ组髓鞘成分有残留;Ⅰ组和Ⅱ组神经管道完整性比正常神经管道结构完整性差,在去细胞神经的制备过程中,应考虑基底膜的活性、去细胞、脱髓鞘及结构完整性的程度。[结论]对于粗大神经的处理,Triton-100、脱氧胆酸钠法作去细胞神经处理可能是一种较好的方法。[Objective]To compare two preparing procedures for larger chemically acellular nerve allografts （CANA）. [Methods]The sciatic nerves of pigs were exposed by a muscle-splitting incision and were isolated free of the underlying fascia. The 60-mm-long segments of the nerve were obtained. They were treated according to the following decellularization processes.In group I,the nerve segments were treated with 7% Triton-100 solution and 7% sodium deoxycholate for two times.In group II,another protocol was created with the detergents Triton X-200,sulfobetaine-16,and sulfobetaine-10 for two times. The degrees of decellularization,activity of laminin,degrees of demyelination,and integrity of the nerve fiber tube were observed under microscope and were assessed by a scoring system.[Results]In both experimental groups the activity of laminin was present and the degrees of decellularization were complete. As for the demyelization of the nerve segments,the myelin sheath in Group II was partially preserved,but it completely disappeared in Group Ⅰ. The structure of the nerve fiber tube in Groups Ⅰ and Ⅱ were not as integral as that in the normal group.[Conclusion]It may be a better method for the larger CANA,to be treated with TritonX-100 and sodium deoxycholate during the decellularization procedure.

关 键 词：神经移植 周围神经损伤 化学去细胞 

分 类 号：R651.3[医药卫生—外科学]