诊断超声联合微泡对比剂辐照不同时间对兔正常睾丸组织的影响  

Influence of various exposure time of diagnostic ultrasound and microbubble ultrasound contrast agent on normal testicular tissue in rabbits

作　　者：陈林[1,2] 詹维伟[1] 刘振华[1] 姚洁洁[1] 周建桥[1] 陈曼[1] 芮文斌[3] 

机构地区：[1]上海交通大学医学院附属瑞金医院超声科,上海200025 [2]复旦大学附属华东医院超声科,上海200040 [3]上海交通大学医学院附属瑞金医院泌尿外科,上海200025

出　　处：《诊断学理论与实践》2010年第2期161-165,共5页Journal of Diagnostics Concepts & Practice

基　　金：上海市科委自然基金(09411963400)

摘　　要：目的:探讨诊断超声联合微泡对比剂辐照不同时间对兔正常睾丸组织的影响。方法:应用诊断超声与常规剂量的超声微泡对比剂(SonoVue),对12只健康新西兰雄性家兔的双侧睾丸进行持续辐照。家兔行随机分组,按辐照的时间分为对照组(空照组)、5min辐照组、10min辐照组和15min辐照组,每组3只(6只睾丸),辐照组于0、12及24h各辐照1次。辐照结束后,处死家兔并获取双侧睾丸,分析其病理改变、计算Johnsen评分和生殖细胞凋亡指数(AI),测定睾丸组织匀浆中一氧化氮(NO)和丙二醛(MDA)含量、一氧化氮合酶(NOS)和超氧化物歧化酶(SOD)活力等生化指标。结果:①5min辐照组和10min辐照组对睾丸生殖细胞凋亡无明显影响,而15min辐照组生殖细胞凋亡明显增加(P<0.01)。②10min辐照组NO含量明显增加,而MDA含量明显降低(P<0.05)。③15min辐照组NO和MDA含量均增加(P<0.05),SOD活力明显降低(P<0.05)。④各组之间Johnsen评分差异无统计学意义(P>0.05)。结论:诊断超声联合SonoVue微泡辐照睾丸组织可产生多种生物学影响,10min辐照可获得较小的细胞损伤和较佳的超声生物学效应。Objective To investigate various exposure time of diagnostic ultrasound and microbubble ultrasound contrast agent on normal testicular tissue in rabbits. Methods Twelve New Zealand male rabbits underwent exposure to diagnostic ultrasound and microbubble ultrasound contrast agent,and these rabbits were divided into control group,5 min group,10 min group and 15 min group （n=3,each） according to the exposure time. The rabbits underwent exposure once at the time of 0 h,12 h and 24 h. Thereafter the rabbits were sacrificed,and the two testis were examined for pathological changes,assessing the Johnsen score and apoptosis index （AI） of spermatogonia,measuring the biochemical parameters NO,MDA,NOS and SOD activity of testicular tissue. Results ① AI of the 5 min and 10 min groups did not differ significantly from that of control group,but that of the 15 min group increased significantly （P〈0.01）. ② Johnsen score had no significant differences among different groups （P〉0.05）. ③ No significant differences of NO,NOS,MDA and SOD were seen between the 5 min group and control group. However,NO,NOS of the 10 min group increased significantly （P0.05） and MDA decreased significantly when compared with control group （P〈0.05）. ④ NO,NOS and MDA of the 15 min group increased significantly （P〈 0.05）,but SOD decreased significantly when compared with control group （P〈 0.05）. Conclusions Exposure to diagnostic ultrasound and microbubble ultrasound contrast agent can exert multiple biological influences on testicular tissue,and exposure for 10 min only cause minimal testicular cell injury and has better ultrasonic biological effects.

关 键 词：超声 微泡对比剂 辐照 睾丸 

分 类 号：R445.1[医药卫生—影像医学与核医学]