应用多重PCR法快速检测伤寒沙门菌的研究  

Identification of Salmonella enterica serovars typhi by multiplex PCR

作　　者：张小贤[1] 张家敏[1] 黄卫平[1] 杨珺[1] 

机构地区：[1]浙江医学高等专科学校,杭州310053

出　　处：《中国卫生检验杂志》2010年第5期1074-1075,1119,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立快速、特异检测伤寒沙门菌的多重PCR法,应用于临床肠热症的快速诊断。方法:根据GenBank,EMBL及DDBL公布的伤寒沙门菌鞭毛蛋白抗原基因、菌体抗原基因及表面抗原基因的核酸序列,设计出针对fliC-d,rfbE,rfbS及viaB基因的4对特异性引物,采用多重PCR法检测伤寒沙门菌标准株、非伤寒沙门菌标准株及伤寒沙门菌临床菌株。结果:实验结果证实,所有伤寒沙门菌株应用多重PCR法均成功扩增出预先设计的4条特异性目的条带。结论:所建立的多重PCR法可用于快速检测和鉴定伤寒沙门菌,为肠热症的临床诊断及流行病学溯源提供了一种更简便易行的检测技术。Objective：To establish a method by Multiplex PCR for rapid detection of Salmonella enterica serovars Typhi to apply rapid diagnosis for Enteric fever in clinical isolates.Methods：Primers were designed using sequences corresponding to indicated Genbank,EMBL and DDBL nucleotide sequences database accession numbers.Selective amplification of fliC-d,rfbE,rfbS and viaB gene by Multiplex PCR for identification of standard kind and clinical isolates of Salmonella enterica serovars Typhi and no Salmonella enterica serovars Typhi.Results：The results showed that all the clinical isolates examined of Salmonella enterica serovars Typhi accurately identification by this assay.Conclusion：The method by Multiplex PCR can be used for rapid detection and identification of Salmonella enterica Serovars Typhi.It is helpful in clinical diagnosis and epidemiologioal investigation.

关 键 词：伤寒沙门菌 基因 多重PCR 

分 类 号：R378.2[医药卫生—病原生物学]