质粒介导的喹诺酮类耐药基因在社区泌尿系感染大肠埃希菌中的分布  

Distribution of plasmid-mediated quinolone resistance determinants in Escherichia coli from community-acquired urinary tract infections

作　　者：常燕子[1] 李巧云[2,3] 裘莉佩[1] 徐炜烽[1] 张顺[2] 蔡挺[2] 

机构地区：[1]浙江省宁波市医疗中心李惠利医院检验科,浙江宁波315041 [2]浙江省宁波市第二医院,浙江宁波315000 [3]宁波大学医学院,浙江宁波315000

出　　处：《中国卫生检验杂志》2010年第5期1092-1094,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省医药卫生科学研究基金(2009A184)

摘　　要：目的:调查质粒介导的喹诺酮类耐药基因在社区泌尿系感染大肠埃希菌中的分布。方法:收集39株从社区获得性泌尿系感染病人分离的大肠埃希菌,PCR扩增qnrA、qnrB、qnrS、aac(6′)-Ib-cr、qepA等质粒介导的喹诺酮类耐药(PMQR)基因。结果:在39株社区获得性泌尿系感染大肠埃希菌中,PMQR基因携带率为15.4%(6/39)。其中,1株携带qnrB基因(1/39,2.6%),1株携带qnrS基因(1/39,2.6%),4株携带aac(6′)-Ib-cr基因(4/39,10.3%)。PMQR基因携带菌株均为ESBL阳性菌株。所有菌株中未检出qnrA和qepA基因。结论:在社区获得性泌尿系感染大肠埃希菌中,PMQR基因的检出率虽不高,但由于其位于质粒上,易于在细菌间传播,须引起高度关注。Objective：To investigate the distribution of plasmid-mediated quinolone resistance determinants in Escherichia coli from community-acquired urinary tract infections.Methods：A total of 39 E.coli isolates from community-acquired urinary tract infections were collected.Plasmid-mediated quinolone resistance（PMQR）genes were amplified,containing qnrA,qnrB,qnrS,aac（6′）-Ib-cr and qepA.Results：Of 39 E.coli isolates,6（15.4%） were PMQR genes carrier.Among them,one isolate had qnrB（1/39,2.6%）,the other one had qnrS（1/39,2.6%）,and four isolates（4/39,10.3%） carried aac（6′）-Ib-cr gene.All these PMQR positive isolates were ESBL-producing ones.QnrA and qepA genes were not present in any of the isolates.Conclusion：The prevalence of PMQR genes is not high in Escherichia coli from community-acquired urinary tract infections.However,they exist in plasmids and were easy to spread to other bacteria,so more attention should be paid to them.

关 键 词：社区获得性泌尿系感染 大肠埃希菌 质粒介导的喹诺酮耐药 

分 类 号：R277[医药卫生—中医学]