孤啡肽对大鼠离体心脏功能的调节作用及其对去甲肾上腺素诱发心脏功能变化的影响  

Effects of orphanin FQ On simple and norepinephrine-activated cardiac function in isolated rat heart

作　　者：韩毅[1] 赵艳丽[1] 郭政[1] 

机构地区：[1]山西医科大学麻醉系山西医科大学第二医院麻醉科,太原市030001

出　　处：《中华麻醉学杂志》2010年第3期313-316,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30772083）

摘　　要：目的 观察孤啡肽（OFQ）对健康大鼠离体心脏功能的调节作用及其对去甲肾上腺素（NE）诱发心脏功能变化的影响.方法 健康成年雄性SD大鼠60只,月龄3-4月,体重250～280g,随机分为10组（n=6）：对照组（C组）、NE组（NE 10^-3 mol/L）、OFQ1组、OFQ2组和OFQ3组（OFQ 10^-6、10^-7和10^-8mol/L）、OFQ＋OFQ受体拮抗剂（UFP）组（OFQ 10^-6mol/L和UFP-101 10^-6mol/L）、NE＋OFQ1组、NE＋OFQ2组和NE＋OFQ3组（NE 10^-5 mol/L和OFQ 10^-4、10^-7和10^-8 mol/L）、NE＋OFQ＋UFP组（NE 10^-5mol/L、OFQ 10^-6mol/L和UFP-101 10^-6mol/L）.麻醉后分离大鼠心脏,采用Langendorff装置灌流离体心脏,记录给药10 min时左室收缩压（LVSP）、左室舒张末期压（LVEDP）、左室发展压（LVDP=LVSP-LVEDP）、HR、左室最大收缩速率（＋dp/dtmax）和左室最大舒张速率（-dp/dtmax）.结果 与C组比较,OFQ1组、OFQ2组和OFQ3组LVSP、LVDP和±dp/dtmax升高,LVEDP和HR降低（P〈0.05或0.01）.与OFQ1组比较,OFQ2组、OFQ3组和OFQ＋UFP组LVSP、LVDP和±dp/dtmax降低,LVEDP和HR升高（P〈0.05或0.01）.与OFQ2组比较,OFQ3组LVSP、LVDP、±dp/dtmax降低,LVEDP和HR升高（P〈0.05或0.01）.与NE组比较,NE＋OFQ1组、NE＋OFQ2组、NE＋OFQ3组LVSP、LVDP、±dp/dtmax和HR增高,LVEDP降低（P〈0.05或0.01）.与NE＋OFQ1组比较,NE＋OFQ2组、NE＋OFQ3组和NE＋OFQ＋UFP组LVSP、LVDP、4-dp/dtmax和HR明显降低,LVEDP升高（P〈0.01）.与NE＋OFQ2组比较,NE＋OFQ3组LVSP、LVDP、±dp/dtmax和HR降低,LVEDP升高（P〈0.01）.结论 OFQ对健康大鼠离体心脏具有正性变力作用,负性变时作用;OFQ可以增强NE诱发心脏的正性变力变时作用.Objective To investigate the effects of orphanin FQ （OFQ） on simple and norepinephrineactivated cardiac function in isolated rat heart.Methods Male SD rats aged 3-4 months weighing 250-280 g were anesthetized with intraperitoneal 25% urethane 1.4-1.8 ml.Their hearts were excised and passively perfused with K-H solutionin a Lanngendorff apparatus.A fluid-filled latex balloon was inserted into left ventricle for measurement of developed pressure.Left ventricular systolic pressure（LVSP）,left ventricular end-diastofic pressure（LVEDP）,left ventricular developed pressure（LVDP=LVSP-LVEDP）,HR and±dp/dtmax were measured.Sixty isolated rat hearts were randomly divided into 10 groups（n=6 each）：Ⅰ control group （group C）;Ⅱ,Ⅲ,Ⅳ OFQ1,OFQ2,OFQ3 group--OFQ 10^-6,10^-7,10^-8 mol/L were administered via perfusion system respectively;Ⅴ OFQ＋UFP group--OFQ 10^-6 mol/L＋UFP-101 （all OFQ antagonist） 10^-6 mol/L were administered;Ⅵ NE group--norepinephrine 10^-5 mol/L;Ⅶ,Ⅷ,Ⅸ NE＋OFQ groups--NE 10^-5 mol/L＋OFQ 10^-6,10^-7,10^-8 mol/L were administered respectively;Ⅹ NE＋OFQ＋UFP group--NE 10^-5 mol/L＋OFQ 10^-6mol/L＋UFP-101 10^-6mol/L.Results LVSP,LVDP and±dp/dtmax were significantly increased while HR was significantly decreased in the 3 OFQ groups as compared with control group.LVSP,LVDP±dp/dt max and HR were significantly increased in the 3 NE＋OFQ groups as compared with NE group.Conclusion OFQ has positive inotropic action and negative chronotropic effect on the isolated rat heart.OFQ Can enhance the positive inotropic and chronotropic effect of NE on the heart.

关 键 词：阿片样肽类 心脏功能实验 去甲肾上腺素 

分 类 号：R285.5[医药卫生—中药学]