异氟烷对发育期大鼠海马IL-1β mRNA、IL-6 mRNA和TNF-α mRNA表达的影响  

Effect of isoflurane on expression of IL-1β mRNA,IL-6 mRNA and TNF-α mRNA In the hippocamapus of immature rats

作　　者：施庆余[1] 罗爱林[1] 李世勇[1] 

机构地区：[1]华中科技大学同济医学院同济医院麻醉科,武汉市430030

出　　处：《中华麻醉学杂志》2010年第3期324-326,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30772086）

摘　　要：目的 探讨异氟烷对发育期大鼠海马IL-1β mRNA、IL-6 mRNA和TNF-α mRNA表达的影响.方法 出生7 d的SD大鼠64只,随机分为2组（n=32）：对照组（C组）和异氟烷麻醉组（I组）.I组大鼠吸入1.5%异氟烷麻醉6 h,C组吸入空气.I组于麻醉前（T0,基础状态）、麻醉2 h（T1）、4 h（T2）、6 h（T3）、麻醉停止后4 h（L）、6 h（T5）、12 h（T6）、24 h（T7）时,C组在对应时点各取4只大鼠,测定海马IL-1β mRNA、IL-6 mRNA及TNF-α mRNA的表达水平.结果 C组各时点海马IL-1β mRNA、IL-6 mRNA和TNF-α mRNA比较差异无统计学意义（P＞0.05）;与C组比较,I组海马T1～5 时IL-1βmRNA表达上调,T2,3 时IL-6 mRNA表达上调,T1～6时TNF-α mRNA表达上调（P＜ 0.05）.结论 1.5%异氟烷麻醉可短暂上调发育期大鼠海马IL-1β mRNA、IL-6 mRNA和TNF-α mRNA的表达.Obiective To investigate the effect ofisoflurane on expression of IL-1β mRNA,IL-6 mRNA and TNF-α mRNA in the hippocampus of immature rats.Methods sixty-four 7-clay-old SD rats were randomly assigned into 2 groups（n=32 each）：control group（group C）and isoflurane group（group S）.group S was exposed to 1.5% isoflurane for 6 h while group C to air.Fore animals were killed before anesthesia（T0,baseline）,at 2,4,6 h（T1-3）of isoflurane anesthesia and 4,6,12 and 24 h after anesthesia（T4-7）.The hippocampi were immediately removed for determimation of the expression of IL-1β mRNA,IL-6 mRNA and TNF-α mRNA by RT-PCR.Results Compared with group C,the expression of IL-1β mRNA at T1-5,IL-6 mRNA at T2.3 and TNF-α mRNA at T1-6 in the hippocampus was upregulated in group S.Conclusion The expression of IL-1β mRNA,IL-6 mRNA and TNF-β mRNA was elevated in the hippocampus of immature rats after being exposed to isoflurane.

关 键 词：异氟醚 白细胞介素类 肿瘤坏死因子Α 

分 类 号：R614[医药卫生—麻醉学]