脊髓TNF-α在小鼠骨癌痛发生中的作用  

Role of spinal cord TNF-α in the development of bone cancer pain in mice

作　　者：高勤[1] 顾小萍[1] 张娟[1] 周晓芳[1] 王俊华[1] 马正良[1] 

机构地区：[1]南京大学医学院附属鼓楼医院麻醉科,210008

出　　处：《中华麻醉学杂志》2010年第3期331-333,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30872439/c160202）;卫生部课题资助项目（WKJ2006-2-8）;江苏省科教兴卫工程重点人才课题（RC2007012）

摘　　要：目的 探讨脊髓TNF-α在小鼠骨癌痛发生中的作用.方法 雄性C3H/He小鼠72只,体重18～25 g,周龄4～6周,随机分为3组（n=24）：假手术组（S组）、骨癌痛组（BCP组）和TNF-α拮抗剂依那西普组（E组）.采用股骨远端骨髓腔注射肿瘤细胞的方法制备小鼠骨癌痛模型,S组注射等量不含肿瘤细胞的α-MEM,E组于注射肿瘤细胞前3 d、注射前即刻、注射后3、6 d时分别腹腔注射依那西普100μg（溶于0.5 ml生理盐水）.分别于注射肿瘤细胞前、注射后3、5、7、10、14 d（T1～6）时测定热痛阈和机械痛阈.分别于T4～6时随机取8只小鼠测定痛阈后处死取脊髓,采用RT-PCR法检测TNF-αmRNA的表达水平.结果 与S组比较,BCP组T3～6时热痛阈和T5、6时机械痛阈降低,T4～6时TNF-αmRNA表达上调,E组T4～6时热痛阈降低,T5,6时机械痛阈降低、TNF-αmRNA表达上调（P〈0.05）;与BCP组比较,E组T4～6时热痛阈、T6时机械痛阚升高,T5,6时TNF-α mRNA表达下调（P〈0.05）.结论 脊髓TNF-α参与了小鼠骨癌痛的发生.Objective To investigate the role of spinal cord TNF-a in the development of bone cancer pain in mice. Methods Seventy-two 4-6 week old C3H/He mice weighing 18-25 g were randomly divided into 3 groups （n = 24 each） ： group I sham operation （group S） ; group II bone cancer pain （group BCP） and group Ⅲ etanercept （group E）. Bone cancer pain was induced by implantation of osteosarcoma NCTC 2472 cells into the intramedullary space of right femur in group II and Ⅲ . Group Ⅲ received intraperitoneal etanercept 100 μg at 3 days before and immediately before and day 3 and 6 after tumor cell inoculation. In group S culture medium α-MEM containing no cancer cell was injected instead. The paw withdrawal threshold to mechanical stimuli （PWMT） and paw withdrawal latency to thermal stimuli （ PWTL） were measured before inoculation （baseline） and at day 3, 5,7, 10, 14 after inoculation respectively. Eight animals were killed on the 7th, 10th, and 14th day after inoculation in each group. The spinal cords were removed and TNF-α mRNA expression in the spinal cord was determined by RT-PCR. Results Cancer pain was significantly attenuated by pretreatment with etanercept. The TNF-α mRNA expression in the spinal cord was significantly increased after inoculation and was significantly attenuated by pretreatment with etanercept in group Ⅲ . Conclusion Spinal cord TNF-a is involved in the development of bone cancer pain in mice.

关 键 词：肿瘤坏死因子A 骨肿瘤 脊髓 

分 类 号：R285.5[医药卫生—中药学]