金线吊乌龟茎叶中生物碱的研究  

Alkaloids in stems and leaves of Stephania cepharantha

作　　者：何丽[1,2] 张援虎[3] 唐丽佳[3] 宋少辉[3] 孙黔云[3] 

机构地区：[1]贵州师范大学化学与材料科学学院 [2]中国科学院天然产物化学重点实验室,贵州贵阳550002 [3]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002

出　　处：《中国中药杂志》2010年第10期1272-1275,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2009CB526500);贵州省中药现代化项目(黔科合中药专字[2006]5041号)

摘　　要：目的:研究金线吊乌龟茎叶中的生物碱类成分。方法:金线吊乌龟干燥茎叶粉末用95%乙醇溶液渗滤提取,减压回收乙醇,依次用石油醚和氯仿萃取,所得氯仿层浸膏利用微孔吸附树脂、正相、反相硅胶、葡聚糖凝胶、聚酰胺等柱色谱方法分离和纯化,通过理化性质及光谱方法鉴定化合物的结构。结果:从金线吊乌龟茎叶中分离得到11个生物碱类成分,分别鉴定为观音莲明(1),四氢巴马亭(2),巴马亭(3),isocorydione(4),紫堇单酚碱(5),紫堇根碱(6),sinoracutine(7),青风藤碱(8),顶花防己胺(9),异紫堇定(10)和紫堇定(11)。结论:化合物2～7为首次从金线吊乌龟中分离得到,且化合物7为首次从千金藤属植物中分离得到,化合物4为首次从防己科植物中分离得到。Objective： To study the alkaloids in the stems and leaves of Stephania cepharantha.Method： The dried stems and leaves of S.cepharantha were percolated with 95% ethanol and the solvent was removed by rotary evaporation to give a concentrate,and the concentrate was extracted by petroleum ether and chloroform.Column chromatograghy on MCI CHP 20P,silica gel,Rp-18,Sephadex LH-20 and polyamide were applied for the isolation and purification of the chloroform fraction.The structures were elucidated by their physicochemical properties and spectral data.Result： Eleven alkaloids were obtained and identified as lysicamine（1）,tetrahadropalmatine（2）,palmatine（3）,isocorydione（4）,corydalmine（5）,corypalmine（6）,sinoracutine（7）,sinoacutine（8）,cepharamine（9）,isocorydine（10） and corydine（11）.Conclusion： Compounds 2-7 were isolated from S.cepharantha for the first time,and compound 7 was isolated from the genus Stephania for the first time,compound 4 was isolated from the Menispermaceae family for the first time.

关 键 词：金线吊乌龟 生物碱 isocorydione 

分 类 号：R284.1[医药卫生—中药学]