辛开苦降法对功能性消化不良大鼠胃舒缩活动的影响  

Effect of TCM Treatment with the Xinkai Kujiang Method on Gastric Motion in Functional Dyspepsia Rats

作　　者：王奕[1] 郝建军[2] 魏玮 田俊[4] 

机构地区：[1]中华中医药学会 [2]武汉市中西医结合医院 [3]北京市宣武区中医医院 [4]华中科技大学同济医学院

出　　处：《中医杂志》2010年第5期450-452,共3页Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局中医药科技专项基金资助(04-05JP11);首都医学发展基金资助项目(2005-SF-I-013)

摘　　要：目的观察辛开苦降法对功能性消化不良(FD)模型大鼠胃舒缩活动的影响。方法将24只大鼠随机分为空白组、模型组和胃康宁组,每组8只,适应性喂养7天后,按照夹尾刺激法制备FD模型,造模7天后,胃康宁组大鼠按0.625g/kg体重灌服胃康宁胶囊,每日2次,其他两组给予同体积生理盐水,连续给药12天,于第13天留取血清和胃组织,测定血浆胃动素(MTL)、一氧化氮(NO)含量及胃体组织MTL、一氧化氮合酶(NOS)、Cajal间质细胞(ICC)和胃肠平滑肌细胞(SMC)的表达。结果模型组血浆MTL水平较空白组明显下降,NO水平明显升高,胃肌间神经丛MTL、ICC及SMC的阳性神经元的表达明显减弱,NOS阳性神经元表达明显增加(P<0.01);与模型组比较,胃康宁组血浆MTL水平明显升高,NO水平明显降低,胃组织MTL、ICC及SMC的表达明显增强,NOS的表达减少(P<0.01)。结论辛开苦降法对FD大鼠的治疗作用与其调节胃舒缩活动有关。Objective To study the effect and mechanism of Xinkai Kujiang method（TCM treatment with the pungent and bitter herbs to regulate the gastrofunctions）on the functional dyspepsia（FD）rats.Methods Twenty-four rats were randomized into control group,model group,and Weikangning（herbal medicine for dyspepsia）group,with 8 in each.After a 7-day adaptive feed,the FD models were made with the clipping tail stimulation.Seven days after modeling,the Weikangning group was given Weikangning Capsule 0.625g/kg by gavage,twice a day,and the other two groups were given normal saline in the same dosage.The medication was appliey in succession for 12 days,and on the thirteenth day,the serum and gastric tissues were collected for detecting the lab indications：plasma motilin（MTL）,plasma nitric oxide（NO）,gastric tissue MTL,nitric oxide synthase（NOS）,and the expression of Cajal interstitial cells（ICC）and gastrointesinal smooth muscle cells（SMC）.Results Compared with the control group,the level of plasma MTL in the model group was significantly decreased,the level of NO significantly increased,and the expression of MTL,ICC and SMC positive neurons in the stomach myenteric nerve plexus was significantly weakened,but the expression of NOS positive neurons was significantly strengthened（P0.01）.Compared with the model group,the level of plasma MTL in the Weikangning group was significantly increased,the level of NO significantly decreased,and the expression of MTL,ICC and SMC of the gastrotissues was significantly increased,but the expression of NOS was decreased（P0.01）.Conclusion The effect of Xinkai Kujiang method on functional dyspepsia rats is related to the adjustment of gastrointestinal movement.

关 键 词：辛开苦降法 功能性消化不良 胃肠运动 胃动素 一氧化氮 

分 类 号：R259[医药卫生—中西医结合]