GAP-43在Ⅰ型糖尿病早期大鼠脑海马中的表达变化  

Expression changes of GAP-43 in the hippocampus of diabetic rats

作　　者：王金德[1] 段艳萍[2] 宋巍[3] 孙霞[3] 李睿[3] 李晓霞[3] 李玲[4] 郭泽云[2] 

机构地区：[1]昆明医学院解剖学教研室,昆明650031 [2]昆明医学院组胚教研室,昆明650031 [3]昆明医学院基础医学院学生,昆明650031 [4]昆明医学院药物实验室,昆明650031

出　　处：《神经解剖学杂志》2010年第1期69-72,共4页Chinese Journal of Neuroanatomy

基　　金：云南省教育科学规划课题(GY09006);昆明医学院本科生科研立项基金(KMY0807)

摘　　要：目的:观察链脲佐菌素(STZ)诱导的糖尿病大鼠早期海马生长相关蛋白(GAP-43)的变化,探讨GAP-43在糖尿病脑病发病中的重要作用。方法:本实验将SD雄性大鼠20只随机分为正常组及糖尿病组,用STZ复制糖尿病动物模型,饲养5d后测血糖,4周后进行灌注固定,应用免疫组织化学方法观察糖尿病组和正常组的大鼠海马CA1、CA2、CA3和CA4区GAP-43的表达情况。结果:糖尿病组大鼠的海马各区GAP-43的表达明显增高,尤以CA1和CA3区的表达增高最为明显,与正常组比较有显著性差异(P<0.05)。结论:结果表明,在糖尿病早期海马内GAP-43已发生了变化,特别是与记忆有关的CA1和CA3区,提示GAP-43的改变可能与糖尿病脑病的发病密切相关。Objective：To observe the expressional change of GAP-43 in the hippocampus of Diabetes Mellitus rats,and study the influence of GAP-43 promotion neurons regeneration after DM in the hippocampus.Methods：Twenty SD male rats were randomly divided into two groups：normal control group and STZ DM group.Five days later,blood glucose was measured,and 4 weeks later,the immunohistochemical methods was used to detect changes of GAP-43 in the hippocampus.Results：GAP-43 in the hippocampus of the DM group was higher than those in the normal group（P〈0.05）.Especially,GAP-43 in the CA1 and CA3 areas were higher than those in the CA2 and CA4 areas.Conclusion：The results indicate that the pathological changes of the hippocampus had occurred early and may played an important role in DM.

关 键 词：糖尿病 海马GAP-43 大鼠 

分 类 号：R587.1[医药卫生—内分泌]