NSE在慢性砷中毒大鼠海马CA3区的表达  

NSE expression of hippocampal CA3 area in rats following chronic arsenic poisoning exposure

作　　者：李玉飞[1] 康朝胜[2] 臧贵勇[2] 李远慧[3] 周启良[1] 胡明华[1] 

机构地区：[1]长沙医学院解剖学教研室,长沙410219 [2]贵阳医学院解剖学教研室,贵阳511700 [3]贵阳医学院组织胚胎学教研室,贵阳511700

出　　处：《神经解剖学杂志》2010年第1期93-97,共5页Chinese Journal of Neuroanatomy

摘　　要：目的:利用神经元特异性烯醇化酶(NSE)免疫组化染色,光镜下观察慢性砷中毒对成年大鼠海马CA3区神经元形态学影响,以及慢性砷中毒对成年大鼠脑的神经毒性。方法:取3～4月龄SD大鼠60只,随机分为正常对照组(自由饮用蒸馏水)、低剂量组(As2O3按4mg/l的比例用蒸馏水配制,自由饮用)和高剂量组(As2O3按100mg/l的比例用蒸馏水配制,自由饮用)。每组20只,各组均用普通饲料喂养,连续喂养4月后取大鼠海马组织,常规石蜡切片尼式染色(Nissl),并利用神经元特异性烯醇化酶(NSE)抗体进行SABC法免疫组化染色,观察大鼠海马CA3区神经元的形态学改变,进行细胞形态学计数及测量NSE阳性反应产物的平均光密度。结果:砷中毒组神经元数量减少,细胞形态不规则,可见大量坏死的神经细胞,NSE免疫组化染色阳性细胞减少(P<0.01),阳性反应产物平均光密度降低(P<0.01)。结论:慢性砷中毒可以导致大鼠海马CA3区神经元结构损伤。Objective：Epidemiological investigations indicate that chronic arsenic exposure can damage brain function in adult.The present study was aimed to explore the neurotoxic effects of arsenic exposure from drinking water on adult rats.Methods：A total of 60 Sprague-Dawley rats of 3-4 months old were randomized into three groups of twenty rats each：rats in control group drank regular water,rats in other groups drank water with final arsenic concentration of 4 mg/l（low-dose group）and 100 mg/l（high-dose group）,respectively,for 4 months.Samples of hippocampus were collected for Nissl staining to observe the structure of hippocampal CA3 area in rat.Computer assisted analysis of digitized coloured microscopic images was applied to determine neuron-specific enolase（NSE）immunohistochemical expression and detect average optical density of NSE positive cells.Results：The Nissl-stained sections reveal remarkable neuron loss,denaturation and necrosis and the immunohistochemical staining showed a reduction both in NSE positive number（P0.01）and average optical density（P0.01）in chronic arsenic exposure groups.Conclusion：Our data suggest that chronic arsenic exposure can cause structural change of neurons and NSE expression reduction in hippocampal CA3 area.

关 键 词：砷中毒 神经元特异性烯醇化酶 免疫组化 海马 

分 类 号：R599[医药卫生—内科学]