Association analysis of genetic polymorphisms and potential interaction of the osteocalcin （BGP） and ER-α genes with body mass index （BMI） in premenopausal Chinese women  

作　　者：Hong XU Wen XIAO Dan LUO Yong-ming LIU Lin ZOU Hai-bin KUANG 

机构地区：[1]Department of Physiology, School of Medicine, Nanchang University, Nanchang 330006, China [2]Department of Pathology, School of Medicine, Nanchang University, Nanchang 330006, China [3]Department of Psychology, the Center of Psychological Rehabilitation, Nanchang 330006, China [4]Department of Radioimmunology, People's Hospital of Jiangxi Province, Nanchang 330006, China

出　　处：《Acta Pharmacologica Sinica》2010年第4期455-460,共6页中国药理学报（英文版）

基　　金：AcknowledgementsThis work was supported by the National Natural Science Foundation of China （No 30760071, 30960118）, the Foundation of the Education Department of Jiangxi Province （No GJJ08106）, and the Foundation of Nanchang University （No J06）. We thank all the study subjects for their participation in the study.

摘　　要：Aim： To investigate whether estrogen receptor α （ER-α） PvuII and osteocalcin （also known as bone Gla protein, or BGP） HindIII genetic polymorphisms and their potential interactions are associated with body mass index （BMI） variation. Methods： Data on BMI and ER-α PvuII and BGP HindIII genotypes were obtained from 328 healthy premenopausal Chinese women in east China . The study subjects were unrelated, at least 21 years old （mean age of 33.2±5.9 years）, and had an average BMI of 21.58±2.59. All subjects were genotyped at the ER-α PvuII and BGP HindIII loci using polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP）. Results： The BGP HindIII genotypes were significantly associated with BMI （P=0.003）. Carriers of the HH and Hh genotypes had approximately 2.73% and 1.27% higher BMI than those of the hh genotype, respectively. In contrast, the ER-α PvuII polymorphism was not significantly associated with BMI （P=0.454）. In addition, there was no evidence of potential interactions between the ER-α and BGP genes in our subjects （P≥0.013）. Conclusion： The HindIII polymorphism of the BGP gene, but not the PvuII polymorphism of the ER-α gene or their potential interaction, was associated with BMI in premenopausal Chinese women.Aim： To investigate whether estrogen receptor α （ER-α） PvuII and osteocalcin （also known as bone Gla protein, or BGP） HindIII genetic polymorphisms and their potential interactions are associated with body mass index （BMI） variation. Methods： Data on BMI and ER-α PvuII and BGP HindIII genotypes were obtained from 328 healthy premenopausal Chinese women in east China . The study subjects were unrelated, at least 21 years old （mean age of 33.2±5.9 years）, and had an average BMI of 21.58±2.59. All subjects were genotyped at the ER-α PvuII and BGP HindIII loci using polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP）. Results： The BGP HindIII genotypes were significantly associated with BMI （P=0.003）. Carriers of the HH and Hh genotypes had approximately 2.73% and 1.27% higher BMI than those of the hh genotype, respectively. In contrast, the ER-α PvuII polymorphism was not significantly associated with BMI （P=0.454）. In addition, there was no evidence of potential interactions between the ER-α and BGP genes in our subjects （P≥0.013）. Conclusion： The HindIII polymorphism of the BGP gene, but not the PvuII polymorphism of the ER-α gene or their potential interaction, was associated with BMI in premenopausal Chinese women.

关 键 词：body mass index （BMI） osteocalcin （BGP） gene estrogen receptor-α （ER-α） gene analysis of covariance （ANCOVA） 

分 类 号：Q579.13[生物学—生物化学] Q987