艾芬地尔对大鼠局灶性脑缺血损伤后脑水肿和AQP4表达的影响  

The effect of ifenprodil on cerebral edema and AQP4 expression after the focal brain ischemia reperfusion injury in rat

作　　者：孟飞[1] 衣服新[1] 叶志军[1] 贾媛媛[2] 

机构地区：[1]辽宁医学院附属一院神经外科,锦州121001 [2]辽宁医学院生理学教研室,锦州121001

出　　处：《神经解剖学杂志》2010年第2期192-196,共5页Chinese Journal of Neuroanatomy

摘　　要：目的:研究大鼠脑缺血后水通道蛋白4(AQP4)在脑内的表达及N-甲基-D-天冬氨基酸(NMDA)受体拮抗剂艾芬地尔对其的调节作用。方法:线栓法制作大脑中动脉阻塞(middle cerebra lartery occlusion,MACO)局灶性脑缺血模型;干湿重法测量脑组织含水量以评估脑水肿的程度;免疫组化,免疫蛋白印记法测量AQP4(水通道蛋白4)的表达。结果:与假手术组比较,模型组和艾芬地尔组脑组织含水量、梗死灶周围AQP4表达明显增加;与模型组比较,艾芬地尔组能显著减少脑组织含水量,减轻梗死灶周围AQP4表达,差异有统计学意义。结论:缺血损伤后脑组织的AQP4表达上升,脑水肿明显,给予艾芬地尔后可抑制AQP4的表达,减轻脑水肿。Objective：To study the effect of ifenprodil,a N-methyl-D-aspara gine （NMDA） receptor antagonist,on brain edema after transient brain focal ischemia in the rats. Methods：The transient focal isehemia was induced by middle cerebral artery occlusion （MCAO）; Brain water content （BWC） was measured by dry-wet weighing method; The expression of aquaporin 4 （AQP4） was assessed by immunocytochemistry and Western Blot technique. Results：Compared with sham operation group,BWC and the expression of AQP4 increased obviously 6 h after MCAO in model group and treatment group; BWC and the expression level of AQP4 in ifenprodil treatment group were remarkably less than that in the model group at same time point. Conclusion：Ifenprodil can reduce brain edema through refraining the expression of AQP4 in transient brain focal ischemia in the rats.

关 键 词：艾芬地尔 脑缺血 脑水肿 水通道蛋白4 

分 类 号：R96[医药卫生—药理学]