角化细胞生长因子及受体在鼓膜穿孔邻近皮肤的研究  

Investigation of KGF and KGFR expression in external auditory canal skin adjacent to tympanic membrane perforation

作　　者：方练[1] 林刃舆[1] 刘继丽[1] 洪娟[1] 

机构地区：[1]温州医学院附属第一医院耳鼻咽喉科,浙江温州325000

出　　处：《中国耳鼻咽喉头颈外科》2010年第4期201-203,共3页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的观察鼓膜穿孔部位邻近皮肤中角化细胞生长因子(keratinocyte growth factor,KGF)及其受体角化细胞生长因子受体(keratinocyte growth factor receptor,KGFR)的表达情况,分析其在慢性化脓性中耳炎不同转归中的作用。方法应用免疫组化SP染色方法和多媒体图像分析系统,观察20例继发性胆脂瘤型中耳炎鼓膜穿孔部位邻近皮肤、胆脂瘤上皮以及耳道深部正常皮肤的KGF和KGFR表达,并和20例非胆脂瘤型中耳炎鼓膜穿孔部位邻近皮肤作对比。结果胆脂瘤型中耳炎鼓膜穿孔邻近皮肤KGF和KGFR的阳性表达率分别为(33.135±6.364)%和(19.965±10.570)%,介于胆脂瘤上皮与耳道正常皮肤之间,明显高于非胆脂瘤型中耳炎患者相应部位(19.380±2.827)%和(13.145±7.935)%。结论KGF和KGFR在慢性化脓性中耳炎不同部位的表达依次上升,在胆脂瘤型中耳炎鼓膜穿孔邻近皮肤的表达明显高于非胆脂瘤型中耳炎,说明胆脂瘤型中耳炎该处皮肤增生更活跃。OBJECTIVE To investigate the continuous expression of keratinocyte growth factor（KGF）and keratinocyte growth factor receptor（KGFR）in external auditory canal skin adjacent to tympanic membrane perforation and analyze the role of KGF and KGFR in the different turnover of chronic otitis media.METHODS The external auditory canal skin adjacent to tympanic membrane perforation from 20 cases with cholesteatoma otitis media and the corresponding cholesteatoma tissue and normal external ear skin were examined by immunohistochemical S-P method and quantitative analysis.The positive rate was compared with 20 cases of non-cholesteatom otitis media＇s external auditory canal skin adjacent to tympanic membrane perforation.RESULTS The staining for KGF and KGFR in cholesteatoma otitis media＇s external auditory canal skin adjacent to tympanic membrane perforation was consistently stronger than that in non-cholesteatoma otitis media.The positive rates of the two tissue was（33.135±6.364）% and（19.965±10.570）%,（19.380±2.827）% and（13.145±7.935）% respectively,revealing a significant difference.CONCLUSION The activity of hyperproliferation of the external auditory canal skin adjacent to tympanic membrane perforation in cholesteatoma otitis media is stronger than that of non-cholesteatoma otitis media.KGF and KGFR may play a more important role for hyperproliferation of cholesteatoma.

关 键 词：生长物质 受体 生长因子 中耳炎 化脓性 免疫组织化学 

分 类 号：R764[医药卫生—耳鼻咽喉科]