沙眼衣原体临床分离株的抗菌药物敏感性及基因分型研究  

In vitro susceptibility to 6 antimicrobial agents and genotyping of clinical isolates of Chlamydia trachomatis

作　　者：薛耀华[1] 郑和平[1] 杨斌[1] 周细玉[1] 吴兴中[1] 黄进梅[1] 曾维英[1] 

机构地区：[1]广东省皮肤性病防治中心,广州510500

出　　处：《中华皮肤科杂志》2010年第5期309-311,共3页Chinese Journal of Dermatology

基　　金：广东省医学科研基金（A2007129）

摘　　要：目的探讨广州地区沙眼衣原体临床株对6种抗菌药物的敏感性和基因型分布，以及两者之间的关系。方法细胞培养法分离衣原体临床株，测定6种抗菌药物对沙眼衣原体的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。巢式PCR扩增沙眼衣原体主要外膜蛋白基因（omp1）的VS1-2片段，核酸测序分型。结果346例患者的泌尿生殖道标本接种后，获得76株沙眼衣原体临床分离株，其中40株传代后达到药敏试验所需菌量。药敏试验结果显示克拉霉素、阿奇霉素、罗红霉素、多西环素、四环素和氧氟沙星的MIC50／MIC90（μg／ml）分别为0．008／0．032、0．08／0．16、0．125／0．5、0．032／0．064、0．25／0．5、0．5／1．0。40株共检出7种基因型，其中E型有14株（35％），其次为J型10株（25％）和F型6株（15％）。7种基因型衣原体菌株对阿奇霉素的MIC。完全相同，而罗红霉素、克拉霉素和氧氟沙星的MIC。在不同型别菌株间有1～4倍的差异。结论克拉霉素、多西环素和阿奇霉素显示较强的抗衣原体活性，7种基因型衣原体对阿奇霉素显示一致的敏感性。Objective To assess the vitro susceptibility to 6 antimicrobial agents and genotypes of clinical isolates of Chlamydia trachomatis （Ct） from Guangzhou region. Methods Ct was isolated from clinical specimens by using McCoy cell culture and subjected to propagation. The minimum inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of 6 antimierobial agents （clarithromycin, roxithromyein, azithromycin, doxycycline, tetracycline, ofloxacin） against Ct isolates were determined in McCoy cell cuhure. Nested PCR was performed to amplify the outer membrane protein 1 （ompl） VS1-2 gene followed by sequencing. Results Seventy-six Ct strains were isolated from 346 urogenital specimens, and 40 strains met the requirements for susceptibility testing after serial propagation. The MIC50/MIC90 of clarithromycin, azithromycin, roxithromycin, doxycycline, tetracycline and ofloxaein were as follows： 0.008/0.032, 0.080/0.160, 0.125/0.500, 0.032/0.064, 0.250/0.500 and 0.500/1.000 mg/L. Seven genotypes were observed. The most prevalent genotypes in decreasing order were E （14, 35%）, J （10, 25%）and F （6, 15%）. The MIC50 was consistent for azitbromycin among the 7 genotypes, but varied by 1 - 4 folds for doxycycline, ofloxacin and roxithromycin. Conclusions Clarithromycin, doxycycline and azithromycin exhibit an excellent activity against Ct, and the activity of azithromycin is consistent among the 7 genotypes of Ct.

关 键 词：衣原体 沙眼 基因型 微生物敏感性试验 

分 类 号：R446.5[医药卫生—诊断学]