植物MAPK信号转导组分的细胞定位与选择性剪接  

Cellular Localization of Components of Mitogen-activated Protein Kinase (MAPK) Cascades and Alternative Splicing

作　　者：潘教文[1] 李德全[1] 

机构地区：[1]山东农业大学生命科学学院,作物生物学国家重点实验室,山东泰安271018

出　　处：《中国生物化学与分子生物学报》2010年第5期393-400,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.30871457);教育部长江学者与创新团队发展计划项目(No.IRT0635)资助~~

摘　　要：促分裂原活化蛋白激酶(MAPK)级联途径在真核生物中是高度保守的,由MAPKs,MAPKKs,MAPKKKs组成,通过MAPKKK→MAPKK→MAPK逐级磷酸化传递细胞信号.已有大量研究表明,MAPK在植物响应生物与非生物胁迫,以及植物激素和细胞周期的信号转导中起重要作用.在植物响应各种逆境过程中激活的MAPK基因,细胞内的定位发生动态变化.选择性剪接是真核生物中调节基因表达的重要模式,能够影响蛋白的结合特性、胞内定位、酶的活性、蛋白的稳定性和翻译后的修饰.MAPK基因的选择性剪接能产生不同的转录异型并具有不同的亚细胞定位.本文综述这方面的研究进展.The mitogen-activated protein kinase （ MAPK） cascade is composed of mitogen-activated protein kinases （ MAPKs ）,mitogen-activated protein kinase kinases （ MAPKKs ）,mitogen-activated protein kinase kinase kinases （ MAPKKKs）,which is highly conserved in eukaryotes. The intracellular signals were relayed and amplified through sequential phosphorylations of MAPKKK→MAPKK→MAPK. In plants,numerous studies show that MAPKs play an important role in the signaling of biotic and abiotic stresses,phytohormones,and cell cycle cues. The localization of the MAPK proteins involves dynamic changes after activated during various responses in plants. Alternative splicing is one of the important mechanisms used to regulate gene expression and is employed by all eukaryotes. Alternative splicing affects the binding property, intracellular localization, enzymatic activity, protein stability and posttranslational modification of a large number of proteins. Alternative splicing of MAPK genes generates different transcript variants that produce proteins with different subcellular localizations. In this review, we will discuss recent advances in relation to this aspect.

关 键 词：促分裂原活化蛋白激酶 亚细胞定位 选择性剪接 

分 类 号：Q71[生物学—分子生物学]