离子束介导玉米DNA影响水稻幼苗根系蛋白水解酶的表达  

Ion Beam Transformation of Corn DNA Affect the Proteinase Expression in Rice Seedling Roots

作　　者：姬生栋[1] 袁召[1] 王书玉 薛应征 岳春晖[1] 王知丰[1] 陈鹏[1] 王海莎[1] 侯磊磊[1] 朱德来[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]新乡市农科院,河南新乡453000

出　　处：《中国生物化学与分子生物学报》2010年第5期442-447,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：河南省自然科学基金资助项目(No.092300410082)~~

摘　　要：利用离子束介导法把玉米DNA导入水稻豫粳6号种子胚中,通过复性电泳技术,分析水稻幼苗根系中蛋白水解酶在pH4.5、pH7.0和pH8.5的表达情况.结果表明:(1)在不同pH条件下处理,各蛋白水解酶种类与活性存在差异,酸性、中性、碱性条件下,检到的蛋白水解酶酶带依次增多,酸性条件下酶活性较弱,中性和碱性条件下酶活性较强;(2)在3种pH条件下,离子束辐照处理均有部分蛋白水解酶带缺失,同时,在中性和碱性条件下检出50kD新酶带,说明离子束辐照可影响水稻蛋白水解酶表达;(3)离子束介导玉米DNA水稻幼苗根系中,pH4.5时无新蛋白水解酶酶带检出,pH7.0和pH8.5时均检到多条新酶带且活性较强.说明离子束介导玉米DNA引起水稻幼根蛋白水解酶表达发生变化.Corn DNA was introduced to dry seeds of rice （YuJing-6） by 25 KeV low energy implantation at a dose of 3. 0 × 10^17 N ＋ /cm^2. The proteinases in the roots of rice seedling were analyzed by renaturation electrophroresis at the pH4. 5,pH7. 0 and pH8. 5,respectively. The more types and activities of proteinases were observed to increase with increasing pH value. Ion radiation of rice seedling roots led to lost of protease bands in all pH conditions. A novel 50 kD band was found at the condition of pH7. 0 and pH8. 5,respectively. Some new bands found at the conditions of pH7. 0 and pH8. 5 after implantation gave stronger activities. The data implicated that the expression of proteinases in the seedling rice were variated by the low energy ion beam mediated transformation with the corn DNAs .

关 键 词：离子束 介导 水稻 蛋白水解酶 

分 类 号：Q556.3[生物学—生物化学]