经皮穴位电刺激对跑台运动大鼠血浆氨基酸及中缝背核细胞外液5-HT含量的影响  

Effects of Transcutaneous Electrical Acupoint Stimulation on the Plasma Amino Acid and Extracellular 5-HT in Dorsal Raphe Nucleus of Exercising Rats

作　　者：方剑乔[1] 梁宜[1] 汪存信[1] 邵晓梅[1] 马桂芝[1] 李海燕[1] 

机构地区：[1]浙江中医药大学第三临床医学院,杭州310053

出　　处：《中国运动医学杂志》2010年第3期309-312,320,共5页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金资助项目(30572412);浙江省高校"重中之重"学科建设项目

摘　　要：目的:探讨经皮穴位电刺激(TEAS)对急性跑台运动大鼠血浆氨基酸及中缝背核(DRN)细胞外液5-HT含量的影响。方法:20只雄性SD大鼠行DRN立体定位术后,随机分为运动组(n=10)和TEAS组(n=10)。术后第2天,所有大鼠开始接受适应性运动,TEAS组介入TEAS治疗,取右侧足三里穴进行电针治疗,参数为连续波,频率2Hz,强度5mA,时间30min,每日1次,连续治疗6天。术后第7天,行微透析采样,收集基础液后进行急性跑台运动(先以10m/min的速度运动10min,在随后4min内将跑速调至24m/min并持续运动1h,坡度为0),微透析采样直至运动后5h。记录运动中毛刷刺激频次;采用脑微透析采样技术和高效液相电化学法(HPLC-ECD)连续观察运动前后大鼠DRN细胞外液5-HT水平动态变化;运动后5h时,采用高效液相紫外法(HPLC-UV)检测血浆氨基酸含量。结果:(1)TEAS组毛刷刺激频次显著低于运动组(P<0.05)。(2)与运动组比较,TEAS组大鼠血浆总色氨酸(T-TRP)和支链氨基酸(BCAA)含量变化不明显(P>0.05),而血浆游离色氨酸(f-TRP)含量(P<0.01)、f-TRP/T-TRP(P<0.01)和f-TRP/BCAA比值(P<0.05)显著降低。(3)运动组运动后即刻DRN细胞外液5-HT水平显著高于基础水平(P<0.05),在随后2h内降低,而在运动后3h和4h时又呈升高趋势,显著高于运动后2h时;TEAS组在运动后各时段均低于运动组,尤以运动后5h为显著(P<0.05)。结论:TEAS足三里穴可有效提高大鼠运动能力,可能通过降低血浆TRP游离度和f-TRP转运入脑,进而减少脑内5-HT的部分合成实现的。Objective To observe the effect of transcutanous electrical acupoint stimulation（TEAS）on the exerciseability of rats. Methods After stereotaxis surgery,twenty male adult SD rats were randomly divided into control group（n=10）and TEAS group（n=10）. Two days after surgery,the rats were trained to adapt the treadmill running andTEAS（continuous wave,2Hz,5mA）was applied to right＂Zusanli＂（ST 36）for 30 min,once a day for 6 days. Basicmicrodialysis samples of extracellular serotonin（5-HT）in rats’dorsal raphe nucleus（DRN）were collected 7 days aftersurgery. During experiment,rats were running on treadmill（10m/min for 10min,then gradually increased to 24m/minfor 1 hour,0% grade）. Microdialysis samples were collected continuously until 5 hours after treadmill running. Thefrequency of brush-stimulation during the running was recorded. Meanwhile,plasma amino acid content was examined5 hours after exercise. Results The frequencies of brush-stimulation during the treadmill running in control group andTEAS group were 2.04±0.46 and 0.40±0.080 times per minute,respectively（P〈0.05）. There were no significant differences in plasma total-TRP（T-TRP）and branched chain amino acid（BCAA）contents between two groups（P〉0.05）.However,plasma free-TRP（f-TRP）content,f-TRP/T-TRP ratio and f-TRP/BCAA ratio decreased significantly（P〈0.05）in TEAS group as compared with the control group. Extracellular 5-HT levels in DRN in control group soon afterexercise were higher than their base levels （P〈0.05）,and decreased in sequential two hours,and then increased significantly 3 hours and 4 hours after exercise as compared with that 2 hours after exercise（P〈0.05）. There was aslow decline in the levels of extracellular 5-HT in TEAS group at each time-point after exercise,and significant lowerthan that in control group within 5 hours after exercise（P0.05）. Conclusion TEAS at ＂Zusanli＂（ST36）can effectively improve exercised ability by reducing peripheral free-TRP level an

关 键 词：经皮穴位电刺激 跑台运动 微透析 色氨酸 5-羟色胺 

分 类 号：R87[医药卫生—运动医学]