复方中药安胃汤对慢性萎缩性胃炎模型大鼠TGF-α、COX-2mRNA表达的影响  

Anwei Decoction up-regulates TGF-α mRNA expression but down-regulates COX-2 mRNA expression in the gastric mucosa of rats with chronic atrophic gastritis

作　　者：韦维[1] 林寿宁[1] 黄贵华[2] 朱永苹[1] 万小刚[1] 刘伟峰[1] 朱光辉[1] 张倩[1] 

机构地区：[1]广西中医学院瑞康医院消化内科,广西壮族自治区南宁市530011 [2]广西中医学院一附院消化内科,广西壮族自治区南宁市530022

出　　处：《世界华人消化杂志》2010年第10期1032-1036,共5页World Chinese Journal of Digestology

基　　金：广西自然科学基金资助项目;桂科自No.0728187~~

摘　　要：目的:探讨复方中药安胃汤对实验性慢性萎缩性胃炎大鼠模型TGF-αmRNA、COX-2mRNA表达的影响.方法:采用甲基硝基亚硝基胍(MNNG)慢性萎缩性胃炎大鼠模型,36只清洁级健康雄性Wistar大鼠随机分为病理模型组、安胃汤组、胃复春对照组,共3组.实时定量PCR方法观察复方中药安胃汤对实验性慢性萎缩性胃炎大鼠模型胃黏膜TGF-α、COX-2mRNA表达的影响,对后者进行图像分析及统计学处理.结果:与病理模型组相比,复方中药安胃汤组和胃复春对照组均显著提高TGF-αmRNA/β-actin值(A值:1.54±0.27vs0.78±0.08,1.30±0.15vs0.78±0.08,P<0.01),复方中药安胃汤组优于胃复春对照组(P<0.05).与病理模型组相比,复方中药安胃汤组和胃复春对照组均显著降低COX-2mRNA/β-actin值(A值:0.36±0.04vs0.72±0.13,0.46±0.06vs0.72±0.13,P<0.01),安胃汤组优于胃复春对照组(P<0.05).结论:复方中药安胃汤可能通过增加胃黏膜TGF-αmRNA表达,降低胃黏膜COX-2mRNA表达而起到治疗CAG作用.AIM：To investigate the effect of Anwei Decoction （AD） on the expression of transforming growth factor-α （TGF-α） and cyclooxygenase-2 （COX-2） mRNAs in the gastric mucosa of rats with chronic atrophic gastritis （CAG）.METHODS：Thirty-six healthy rats were randomly divided into three groups：model control group （untreated）,AD treatment group （treated with AD） and Weifuchun treatment group （treated with Weifuchun）.CAG was induced in rats using N-methy-N-nitro-N-nitroguanidine （MNNG）.After treatment,the expression of TGF-α and COX-2 mRNAs in the gastric mucosa of rats was determined by real-time reverse transcription-polymerase chain reaction （RT-PCR）.RESULTS：Compared with the model control group,the expression of TGF-α mRNA was evidently enhanced in the AD treatment group and Weifuchun treatment group （0.78 ± 0.08 vs 1.54 ± 0.27 and 1.30 ± 0.15,respectively,both P〈0.01）.Significant difference was also noted in the expression level of TGF-α mRNA between the two treatment groups （P〈0.05）.In contrast,the expression level of COX-2 mRNA was remarkably reduced in the AD treatment group and Weifuchun treatment group compared with the model control group （0.36 ± 0.04 and 0.46 ± 0.06 vs 0.72 ± 0.13,respectively;both P〈0.01）.Significant difference was also noted in the expression level of COX-2 mRNA between the two treatment groups （P〈0.05）.CONCLUSION：AD exerts therapeutic effects against CAG by enhancing TGF-α mRNA expression and reducing COX-2 mRNA expression in the gastric mucosa.

关 键 词：安胃汤 慢性萎缩性胃炎 转化生长因子-Α 环氧合酶-2 实验研究 

分 类 号：R259[医药卫生—中西医结合]