延胡索乙素对大鼠慢性压迫背根节神经元自发放电的影响  

Effect of 1-Tetrahydropalmatine on Spontaneous Electric Discharge Induced by Chronic Dorsal Root Ganglion Neurons Compression in Rats

作　　者：杜兰芳[1] 杜永平[1] 徐晖[2] 王文挺[2] 胡三觉[2] 

机构地区：[1]第四军医大学西京医院中医科,西安710032 [2]第四军医大学神经生物研究所

出　　处：《中国中西医结合杂志》2010年第4期388-392,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金重点资助项目(No.3053026);国家自然科学基金资助项目(No.30870830)

摘　　要：目的观察延胡索乙素(l-THP)对大鼠慢性压迫背根节神经元A类纤维自发放电的影响。方法采用单纤维记录大鼠慢性压迫背根节神经元自发放电的方法,观察l-THP对自发放电的影响,以及浓度-效应关系。结果实验中记录到单纤维A类神经元自发放电84根。在A类神经元自发放电中有25根自发放电为周期节律,59根为非周期节律。l-THP(100μmol/L)分别引起16.0%(4/25)的周期节律放电神经元和67.8%(40/59)非周期节律放电神经元放电减少(P<0.01,l-THP干预前后配对t检验)。l-THP对受损背根节(DRG)神经元自发放电的抑制作用具有剂量依赖关系,随着浓度的增大抑制神经元自发放电的时间越快,自发放电恢复所需的时间也越长。其中有57.1%(24/42)的DRG神经元自发放电在洗脱后20 min内可以恢复,42.8%(18/42)的DRG神经元自发放电在洗脱后经过3 h始终没有恢复。结论l-THP对大鼠慢性压迫背根节神经元A类纤维的自发放电有抑制作用。Objective To study the effect of 1-tetrahydropalmatine（1-THP） on the spontaneous electric discharge（SED） induced by chronic dorsal root ganglion neurons compression.Methods Using single fiber recording method,the SED of 84 neurons class A induced by compression were recorded.The effect of 1-THP on the SEDs and its relation with concentration were observed.Results In the 84 SED of neurons,25 showed periodical rhythmicity（PR） and 59 showed non-periodic rhythmicity（non-PR）.1-THP（100 μmol/L） inhibited SED in 16.0%（4/25） of neurons with PR and 67.8%（40/59） of neurons with non-PR（P〈0.01） in an effect-dose dependent manner,the higher the concentration of 1-THP,the more the inhibition,with quicker inhibiting initiation and longer time needed for recovery.SED in 57.1% neurons were recovered 20 min after elution,but unrecovered even after 3 h in the others.Conclusion 1-THP shows inhibitory effect on the A-fiber SED induced by chronic dorsal root ganglion neurons compression.

关 键 词：延胡索乙素 自发放电 慢性压迫 背根节 

分 类 号：R285[医药卫生—中药学]