林蛙油含药血清对成骨细胞和破骨细胞增殖和活性的影响  

Effects of serum from oviductus ranae on the proliferation and differentiation of osteoblasts and osteoclast

作　　者：王丹辉[1] 王照辉[1] 吴巍 顾淑珠[3] 朱国英[3] 

机构地区：[1]吉林省中医药科学院,长春130021 [2]北京同仁堂生物制品开发有限公司 [3]复旦大学放射医学研究所

出　　处：《中国骨质疏松杂志》2010年第5期318-321,共4页Chinese Journal of Osteoporosis

基　　金：中国博士后科学基金(20090451502)

摘　　要：目的探讨林蛙油含药血清对成骨细胞(Osteoblast,OB)增殖、分化和矿化能力的影响,及其对破骨细胞(Osteoclast,OC)分化的影响。方法源于大鼠颅盖骨的原代OB中加入不同浓度的林蛙油含药血清进行干预。MTT法观察林蛙油含药血清对OB增殖的影响,用硝基苯磷酸盐(p-nitrophenyl phosphate,PNPP)偶氮法观察林蛙油含药血清对OB碱性磷酸酶(alkaline phosphatase,ALP)活性的影响,茜素红(alizarin red S,ARS)进行矿化结节染色并计算面积以观察林蛙油含药血清对OB矿化能力的影响。RANKL诱导前破骨细胞株RAW264.7细胞6d后,加入林蛙油含药血清,用抗酒石酸酸性磷酸酶(Tartrate Resistant Acid Phosphatase,TRAP)染色法观察TRAP阳性多核细胞。结果林蛙油含药血清可使体外培养OB的增殖率明显提高(P<0.01),并可明显促进OB的矿化能力(P<0.05),但其对OB的ALP活性影响作用不明显(P>0.05)。此外,林蛙油含药血清对RANKL诱导体外培养的破骨前体细胞RAW264.7形成的成熟多核破骨细胞有抑制作用,表现为TRAP(+)成熟多核破骨细胞数明显减少(P<0.01)。结论林蛙油含药血清可促进OB的增殖能力和矿化能力,并可抑制破骨细胞的形成。Objective To investigate the effects of serum from Oviductus Ranae on the proliferation, differentiation and mineralization in osteoblast, and its function on osteoclast differentiation. Methods Serum from Oviductus Ranae with different density were fed to calvaria bone specimens obtained from rat. The proliferation of osteoblasts were analyzed using MTT assay and the ALP activity of OB was observed via PNPP. After being dyed by ARS, the area of calcium nodule was calculated to see the effect of serum from Oviductus Ranae on OB mineralization. 6 days after the pre-OC strain （RAW-264.7） was induced by RANKL, serum from Oviductus Ranae was added, to observe the TRAP activiy. Results Serum from Oviductus Ranae has notable effects on the proliferation of in vitro cultured OB （P 〈0.01 ） and remarkably stimulated the mineralization of OB （P 〈 0.05 ） , while its effect on the ALP activity of OB was not obvious （P 〉 0.05）. Meanwhile, serum from Oviductus Ranae has some inhibitory function on the transformation of pre-OC strain （RAW-264.7） induced by RANKL into mature muhinucleated OC which was shown in significant reduction of the number of TRAP（ ＋ ） （P 〈 0. 01 ）. Conclusion Serum from Oviductus Ranae can promote the proliferation and mineralization ability of OB, and significantly inhibit the formation of OC.

关 键 词：林蛙油 含药血清 成骨细胞 破骨细胞 

分 类 号：R274.9[医药卫生—中西医结合]