儿童哮喘与白三烯受体1及C4合成酶基因多态性的关系  

Cysteinyl leukotrienes receptor 1 and leukotriene C4 synthetase genetic polymorphism in asthmatic children

作　　者：解玉[1] 杨子珍[1] 柴柏春[1] 

机构地区：[1]上海中医药大学附属普陀医院儿科,上海200062

出　　处：《临床医学》2010年第5期40-43,共4页Clinical Medicine

摘　　要：目的探讨白三烯受体1(CYSLTR1)和白三烯C4合成酶(LTC4S)基因多态性与上海儿童哮喘及其严重程度之间的关系。方法 2006年9月至2009年8月本院儿科住院及门诊儿童哮喘患者72例,男39例,女33例,平均年龄(5.1±1.6)岁。根据哮喘发作程度分为间歇和轻度发作组44例、中重度发作组28例。健康对照组成人95例,男50例,女45例。应用连接酶检测反应(LDR)技术,对CYSLTR1的927T/C位点和LTC4S的444A/C位点基因多态性进行分析。结果①哮喘组和对照组927T/C位点基因型、等位基因频率比较(χ2=0.178,0.04,P(0.05)、444A/C位点基因型、等位基因频率比较(χ2=1.14,1.065,P(0.05)差异均无统计学意义。②927T/C和444A/C位点基因型频率在哮喘各组中的分布比较(χ2=0.927,1.138,P(0.05)差异均无统计学意义。结论在上海哮喘儿童中,CYSLTR1的927T/C位点和LTC4S的444A/C位点基因多态性可能不是其发病和严重程度的主要基因危险因素。Objective To observe the relationship between cysteinyl leukotrienes receptor 1（CYSLTR1）and leukotriene C4 synthetase（LTC4S） genetic polymorphism and severity of asthma in asthmatic children of Shanghai area.Methods Seventy-two asthmatic patients hospitalized or in outpatient clinic were included from September 2006 to August 2009.Among them there were 39 males and 33 females with mean age（5.1±1.6） years.According to the severity of asthma the patients were divided into mild intermittent and persistent group（44 patients）,moderate severe group（28 patients）.Ninety-five healthy adults（50 males and 45 females） had also been recruited.The genetic polymorphisms were assessed with ligase chain reaction（LCR） technique.Results ①There was no significant difference in the genotype and allele frequency of 927T/C（χ^20.178,0.04,P﹥0.05）and 444A/C（χ^21.14,1.065,P﹥0.05）between asthma group and control group.②There was no significant difference in the genotype frequency of 927T/C and 444A/C in asthma groups（χ^20.927,1.138,P﹥0.05）.Conclusion In Shanghai asthmatic children,CYSLTR1 927T/C and LTC4S 444A/C genetic polymorphisms might not be associated with the pathogenesis of asthma and severity.

关 键 词：哮喘 白三烯受体1 白三烯C4合成酶 儿童 基因多态性 

分 类 号：R725.6[医药卫生—儿科]