荧光免疫法测定鸡肉中己烯雌酚残留  

Determination of Residual Diethylstilbestrol in Chicken by Fluoroimmunoassay

作　　者：孙梅梅[1] 赵艳伟[1] 梁艳[1] 钱建瑞[1] 李立华[1] 王术皓[1] 

机构地区：[1]聊城大学化学化工学院,聊城252059

出　　处：《理化检验（化学分册）》2010年第5期465-467,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金(20877037);山东省自然科学基金(2005ZX12);山东省泰山学者建设工程基金的资助

摘　　要：用制备的抗己烯雌酚抗体包被酶标板,以双抗夹心免疫响应模式为基础,异硫氰酸荧光素为荧光探针,提出了抗己烯雌酚抗体包被模式荧光免疫法测定鸡肉中己烯雌酚残留量。己烯雌酚的质量浓度的对数值（lgρ）与其荧光强度在0.1-1.0×103μg.L-1范围内呈线性关系,检出限（3S/N）为50 ng.L-1。此法用于鸡肉样品的分析,回收率在85.5%-113.8%之间。The prepared anti-diethylstilbestrol（DES） antibody was used to coat ELISA plates,and isothiocyanic acid fluorescein was used as the fluorescence probe.Based on double-antibody sandwich immune response model,a method for the determination of DES in chicken by fluoroimmunoassay was described.Linear relationship between values of fluorescence intensity and logarithm of mass concentration（lgρ） of DES was obtained in the range of 0.1-1.0×103μg·L-1 with detection limit（3S/N） of 50 ng·L-1.The proposed method was applied to the analysis of samples of chicken,giving values of recovery in the range of 85.5%-113.8%.

关 键 词：荧光免疫分析 己烯雌酚 荧光探针 

分 类 号：O657.3[理学—分析化学]