抑肽酶对脑缺血再灌注大鼠脑组织CD4^+、CD8^+T淋巴细胞表达的影响  

Effect of Trasylol on Expression of CD4^+ and CD8^+ T Lymphocytes in Brain Tissue of Cerebral Ischemia- reperfusion Rats

作　　者：江承平[1] 王晓明[1] 吴碧华[1] 刘福[1] 

机构地区：[1]川北医学院附属医院,南充市637007

出　　处：《中国药房》2010年第21期1962-1964,共3页China Pharmacy

摘　　要：目的:探讨抑肽酶在细胞免疫应答的干预下对脑缺血再灌注后继发性脑损伤的保护作用。方法:105只SD大鼠随机分为假手术组、模型组、抑肽酶组(n=35),采用大鼠大脑中动脉闭塞法制作局灶性脑缺血再灌注模型。抑肽酶组在大鼠脑缺血前24h及再灌注即刻、24h、4d、6d、8d、10d、12d、14d分别尾静脉给予抑肽酶,其余组给予生理盐水。应用免疫组织化学方法检测大鼠脑缺血再灌注不同时间段CD4+、CD8+T淋巴细胞的浸润情况。结果:与假手术组比较,模型组大鼠在缺血再灌注损伤后各时间点脑组织内CD4+、CD8+T淋巴细胞计数均明显增加,10d达高峰;与模型组比较,抑肽酶组大鼠脑组织内CD4+、CD8+T淋巴细胞计数在缺血再灌注损伤后各时间点均更低(P<0.05),8d达高峰。结论:抑肽酶可能通过抑制CD4+、CD8+T淋巴细胞在缺血再灌注损伤后脑组织内的浸润而减少细胞免疫应答介导的神经元凋亡,从而减轻脑组织继发性损伤。OBJECTIVE： To study protection effect of trasylol on cerebral injury resulting from cerebral ischemia-reperfusion in the interference of immunological response.METHODS： 105 SD rats were randomly assigned into sham operation group,model group and trasylol group （n=35）.Focal ischemia-reperfusion model were established by occluding middle cerebral artery.24 h before ischemia and at the 0 h,24 h,4 d,6 d,8 d,10 d,12 d and 14 d of reperfusion,trasylol group were given trasylol via tail vein and other two groups were given normal saline.The infiltration of CD4＋ and CD8＋ T lymphocytes was observed using immunohistochemistry at different stages of cerebral ischemia-reperfusion.RESULTS： As compared with sham operation group,CD4＋and CD8＋ T lymphocytes count in model group were increased significantly after cerebral ischemia-reperfusion injury and obtained maximum value at 10th day.CD4＋ and CD8＋ T lymphocytes count in trasylol group were lower than in model group after cerebral ischemia-reperfusion injury and obtained maximum value at 8th day （P0.05）.CONCLUSION： Trasylol may inhibit the infiltration of CD4＋ and CD8＋ T lymphocytes after cerebral ischemia-reperfusion injury and immunological response-inducing neuron apoptosis so as to relieve secondary cerebral injury.

关 键 词：抑肽酶 脑缺血再灌注损伤 CD4+T淋巴细胞 CD8+T淋巴细胞 大鼠 

分 类 号：R965[医药卫生—药理学] R972[医药卫生—药学]