20(S)-原人参二醇药质体药物含量及包封率的测定  

RP-HPLC determination of 20(S)-protopanaxadiol and the entrapment efficiency of 20(S)-protopanaxadiol in its pharmacosomes

作　　者：陈婧[1,2] 韩美华[1] 季宇彬 王向涛[1] 

机构地区：[1]中国医学科学院药用植物研究所,北京100193 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站,哈尔滨150076 [3]国家教育部抗肿瘤天然药物工程研究中心哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站,哈尔滨150076

出　　处：《中国新药杂志》2010年第10期892-895,共4页Chinese Journal of New Drugs

基　　金：中央级公益性科研院所基本科研业务专项(YZ-1-23);人事部出国留学人员基金[人厅发(2007)170号]

摘　　要：目的：用饱和磷脂制备了20（S）-原人参二醇药质体,并建立20（S）-原人参二醇氢化豆磷脂药质体中20（S）-原人参二醇含量及包封率的测定方法。方法：以氢化豆磷脂为膜材,采用薄膜超声法在65℃超声30 min制备了20（S）-原人参二醇的药质体。采用反相高效液相色谱法,色谱柱为COSMOSIL5 C18-MS-Ⅱ（250 mm×4.6 mm,5μm）;流动相为甲醇-水（95∶5）;流速为1.0 mL.min-1;检测波长为203 nm;柱温为25℃;进样量50μL。结果：药质体易于制备,稳定性好。在上述色谱条件下氢化豆磷脂和试剂对药物的测定无干扰,20（S）-原人参二醇在0.1~0.5 mg.mL-1范围内,PPD浓度与峰面积呈良好的线性关系,r=0.999 9,回收率在103.11%~104.78%之间,日内及日间RSD均小于2%（n=3）。结论：本方法操作简便,结果准确,重现性好,可用于药质体中20（S）-原人参二醇的含量及包封率测定。Objective：To prepare 20（S）-protopanaxadio pharmacosomes with saturated phospholipid,and to establish a RP-HPLC method for both determination of 20（S）-protopanaxadiol and the entrapment efficiency of 20（S）-protopanaxadiol in its pharmacosomes.Methods： By using hydrogenated soybean phospholipids as a membrane material,and ultrasounding for 30min at 65℃ with the method of thin-film ultrasound,20（S）-protopanaxadio pharmacosomes was prepared.The RP-HPLC separation was performed with a COSMOSIL 5C18-MS-Ⅱ column（250mm×4.6mm,5μm） using methanol-water（95∶5） as the mobile phase and detected at 203nm.The flow rate was 1.0mL·min-1 and 50μL sample solution was injected for each time.Results： Using the method,the pharmacosomes was easily prepared and had a good stability.Under the above-mentioned chromatographic conditions,the drug determination was not interfered with hydrogenated soybean phospholipid and reagents.The calibration curve was linear within the range of 0.1~0.5mg·mL-1（r=0.9999）.The average recovery was between 103.11%~104.78%,and both the intra-day RSD and inter-day RSD were less than 2%（n=3）.Conclusion： The method is simple,accurate and sensitive;it is applicable for both determination of 20（S）-protopanaxadiol and the entrapment efficiency of 20（S）-protopanaxadiol in its pharmacosomes.

关 键 词：20(S)-原人参二醇 药质体 包封率 反相高效液色谱法 

分 类 号：R927.2[医药卫生—药学]