人巨细胞病毒pp65DNA疫苗诱导小鼠免疫效应的研究  

Study on the mouse immune responses induced by DNA vaccine with the pp65 gene of human cytomegalovirus

作　　者：杨世炳[1] 唐久来[2] 盛晓蓉 

机构地区：[1]杭州市红十字会医院,浙江310003 [2]安徽医科大学第一附属医院 [3]安徽省医学研究所

出　　处：《中国预防医学杂志》2010年第5期511-512,共2页Chinese Preventive Medicine

基　　金：安徽省十五重大科技攻关专项基金(01303005)

摘　　要：目的探讨人巨细胞病毒pp65 DNA疫苗免疫小鼠产生的细胞免疫应答及相关的细胞因子变化,分析该疫苗是否具高度免疫性。方法将40只健康Balb/c小鼠随机分成A、B两组,每组20只。A组(对照组):注射生理盐水;B组(实验组):注射pp65 DNA疫苗;采用流式细胞仪检测CD3+、CD4+及CD8+;采用双抗体夹心ELISA法检测小鼠血清中的IFN-γ;采用放射免疫法检测小鼠血清中的IL-2。统计学处理用SPSS 10.0统计学软件,组间比较用t检验,P<0.05为差异有统计学意义。结果B组小鼠T细胞亚群CD4+、CD8+、CD3+及细胞因子IFN-γ,IL-2水平明显高于A组,差异均有统计学意义(P<0.01)。结论本研究表明HCMV pp65 DNA疫苗能诱导Balb/c小鼠产生一定的细胞免疫应答及细胞因子的变化。Objective To evaluate cellular immune responses induced by the phosphoprotein 65（pp65） DNA vaccine of human cytomegalovirus（HCMV）in Balb/c mice,and to explore the immunity of the DNA vaccine.Methods Forty female Balb/c mice（aged 6-8 weeks）were randomly divided into vaccination（group B,n=20）and control group（group A,n=20）.Mice in group B were injected with 200 μg recombinant pp65 DNA vaccine at 0,2 and 4 weeks respectively,while mice in group A were injected with same volume of saline at the same schedule.CD3＋,CD4＋ and CD8＋ T cells were measured by flow cytometry（FCM）.The method of double antibody sandwich ELISA and radio-immunity assay（RI）were used to detect serum IFN-γ and IL-2 respectively.Results The number of CD3＋,CD4＋ and CD8＋ T cells as well as the concentration of cytokine IL-2 and IFN-γ were significantly higher in group B mice than those in group A mice（P0.01）.Conclusion The pp65 DNA vaccine of HCMV induced specific cellular immune responses and increased the production of cytokines in Balb/c mice.

关 键 词：细胞免疫 DNA疫苗 人巨细胞病毒PP65 细胞因子 

分 类 号：R392.11[医药卫生—免疫学]